BMRB Entry 15740

Click here to enlarge.

PDB ID: 2k36
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15740
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Structure ensemble Backbone and side-chain 1H, 13C, and 15N Chemical Shift Assignments, 1H-15N RDCs and 1H-1H nOe restraints for protein K7 from the Vaccinia virus
Deposition date:
2008-04-22
Original release date:
2008-11-11
Authors:
Kalverda, Arnout; Thompson, Gary; Vogel, Andre; Schr der, Martina; Bowie, Andrew; Khan, Amir; Homans, Steve
Citation:

Citation: Kalverda, Arnout; Thompson, Gary; Vogel, Andre; Schroeder, Martina; Bowie, Andrew; Khan, Amir; Homans, Steve. "Poxvirus K7 protein adopts a Bcl-2 fold: Biochemical mapping of its interactions with human DEAD box RNA helicase DDX3"  J. Mol. Biol. 385, 843-853 (2009).
PubMed: 18845156

Assembly members:

Assembly members:
K7, polymer, 145 residues, 17489.023 Da.

Natural source:

Natural source:   Common Name: Vaccinia Virus   Taxonomy ID: 10245   Superkingdom: Viruses   Kingdom: not available   Genus/species: Vaccinia Virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
K7: GAMEDAVFYFVDDDKICSRD SIIDLIDEYITWRNHVIVFN KDITSCGRLYKELMKFDDVA IRYYGIDKINEIVEAMSEGD HYINFTKVHDQESLFATIGI CAKITEHWGYKKISESRFQS LGNITDLMTDDNINILILFL EKKLN

Data sets:
Data typeCount
13C chemical shifts612
15N chemical shifts145
1H chemical shifts941
residual dipolar couplings70

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1K71

Entities:

Entity 1, K7 145 residues - 17489.023 Da.

The sequence matches the natural polymer from residue 4 onwards which is equivalent to residue 8 in the natural sequence The GAM n-terminal tag is an expression artifact

1   GLYALAMETGLUASPALAVALPHETYRPHE
2   VALASPASPASPLYSILECYSSERARGASP
3   SERILEILEASPLEUILEASPGLUTYRILE
4   THRTRPARGASNHISVALILEVALPHEASN
5   LYSASPILETHRSERCYSGLYARGLEUTYR
6   LYSGLULEUMETLYSPHEASPASPVALALA
7   ILEARGTYRTYRGLYILEASPLYSILEASN
8   GLUILEVALGLUALAMETSERGLUGLYASP
9   HISTYRILEASNPHETHRLYSVALHISASP
10   GLNGLUSERLEUPHEALATHRILEGLYILE
11   CYSALALYSILETHRGLUHISTRPGLYTYR
12   LYSLYSILESERGLUSERARGPHEGLNSER
13   LEUGLYASNILETHRASPLEUMETTHRASP
14   ASPASNILEASNILELEUILELEUPHELEU
15   GLULYSLYSLEUASN

Related Database Links:

PDB 2K36 3JRV
DBJ BAA00293
EMBL CAA48965 CAA64114 CAB54624 CAM58208 CRL86498
GB AAA48013 AAA60772 AAB96484 AAF33890 AAO89318
PRF 2015436AB
REF NP_042068 YP_232921
SP P68466 P68467 Q76ZX2
AlphaFold P68466 P68467 Q76ZX2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks