BMRB Entry 15074

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PDB ID: 2jmo
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15074
MolProbity Validation Chart

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Title:
Assignment of the 1H, 15N and 13C resonances and determination of the 3D- structure of In-Between-RING (IBR) domain of Parkin
Deposition date:
2006-12-12
Original release date:
2007-05-09
Authors:
Shaw, Gary; Beasley, Steven
Citation:

Citation: Beasley, Steven; Hristova, Ventzislava; Shaw, Gary. "Structure of the Parkin in-between-ring domain provides insights for E3-ligase dysfunction in autosomal recessive Parkinson's disease."  Proc. Natl. Acad. Sci. U. S. A. 104, 3095-3100 (2007).
PubMed: 17360614

Assembly members:

Assembly members:
IBR_domain_of_human_Parkin, polymer, 80 residues, 8610.6 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: p11 (modified pET15b)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IBR_domain_of_human_Parkin: GHMGEEQYNRYQQYGAEECV LQMGGVLCPRPGCGAGLLPE PDQRKVTCEGGNGLGCGFAF CRECKEAYHEGECSAVFEAS

Data sets:
Data typeCount
13C chemical shifts289
15N chemical shifts81
1H chemical shifts475

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1IBR domain of Parkin1
2zinc ion, 12
3zinc ion, 22

Entities:

Entity 1, IBR domain of Parkin 80 residues - 8610.6 Da.

Residues 1-2 are artifacts of a non-native affinity tag

1   GLYHISMETGLYGLUGLUGLNTYRASNARG
2   TYRGLNGLNTYRGLYALAGLUGLUCYSVAL
3   LEUGLNMETGLYGLYVALLEUCYSPROARG
4   PROGLYCYSGLYALAGLYLEULEUPROGLU
5   PROASPGLNARGLYSVALTHRCYSGLUGLY
6   GLYASNGLYLEUGLYCYSGLYPHEALAPHE
7   CYSARGGLUCYSLYSGLUALATYRHISGLU
8   GLYGLUCYSSERALAVALPHEGLUALASER

Entity 2, zinc ion, 1 - Zn - 65.409 Da.

1   ZN

Related Database Links:

PDB 2JMO 4BM9 4I1F 4I1H 5C1Z 5C23 5C9V
DBJ BAA25751 BAF43729 BAF85279 BAG57845 BAI46122
EMBL CAH90914
GB AAM21457 AAM21459 AAM21461 AAS88422 ADB90270
REF NP_001125521 NP_004553 NP_054642 NP_054643 XP_001099588
SP O60260
AlphaFold O60260

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks