BMRB Entry 7427

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7427

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Title:
Yersinia pseudotuberculosis type III secretion effector YopE.
Deposition date:
2008-07-17
Original release date:
2008-08-13
Authors:
Rodgers, Loren; Gamez, Alicia; Riek, Roland; Ghosh, Partho
Citation:

Citation: Rodgers, Loren; Gamez, Alicia; Riek, Roland; Ghosh, Partho. "The type III secretion chaperone SycE promotes a localized disorder-to-order transition in the natively unfolded effector YopE."  J. Biol. Chem. 283, 20857-20863 (2008).
PubMed: 18502763

Assembly members:

Assembly members:
YopE, polymer, 227 residues, 24052.1 Da.
YopE_effector_domain, polymer, 149 residues, 15876.0 Da.

Natural source:

Natural source:   Common Name: Yersinia pseudotuberculosis   Taxonomy ID: 633   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Yersinia pseudotuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
YopE: MKISSFISTSLPLPTSVSGS SSVGEMSGRSVSQQTSDQYA NNLAGRTESPQGSSLASRII ERLSSVAHSVIGFIQRMFSE GSHKPVVTPAPTPAQMPSPT SFSDSIKQLAAETLPKYMQQ LNSLDAEMLQKNHDQFATGS GPLRGSITQCQGLMQFCGGE LQAEASAILNTPVCGIPFSQ WGTIGGAASAYVASGVDLTQ AANEIKGLAQQMQKLLSLML EHHHHHH
YopE_effector_domain: MEGSHKPVVTPAPTPAQMPS PTSFSDSIKQLAAETLPKYM QQLNSLDAEMLQKNHDQFAT GSGPLRGSITQCQGLMQFCG GELQAEASAILNTPVCGIPF SQWGTIGGAASAYVASGVDL TQAANEIKGLAQQMQKLLSL MLEHHHHHH

Data sets:
Data typeCount
15N chemical shifts129
1H chemical shifts129

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1YopE1
2YopE effector domain2

Entities:

Entity 1, YopE 227 residues - 24052.1 Da.

1   METLYSILESERSERPHEILESERTHRSER
2   LEUPROLEUPROTHRSERVALSERGLYSER
3   SERSERVALGLYGLUMETSERGLYARGSER
4   VALSERGLNGLNTHRSERASPGLNTYRALA
5   ASNASNLEUALAGLYARGTHRGLUSERPRO
6   GLNGLYSERSERLEUALASERARGILEILE
7   GLUARGLEUSERSERVALALAHISSERVAL
8   ILEGLYPHEILEGLNARGMETPHESERGLU
9   GLYSERHISLYSPROVALVALTHRPROALA
10   PROTHRPROALAGLNMETPROSERPROTHR
11   SERPHESERASPSERILELYSGLNLEUALA
12   ALAGLUTHRLEUPROLYSTYRMETGLNGLN
13   LEUASNSERLEUASPALAGLUMETLEUGLN
14   LYSASNHISASPGLNPHEALATHRGLYSER
15   GLYPROLEUARGGLYSERILETHRGLNCYS
16   GLNGLYLEUMETGLNPHECYSGLYGLYGLU
17   LEUGLNALAGLUALASERALAILELEUASN
18   THRPROVALCYSGLYILEPROPHESERGLN
19   TRPGLYTHRILEGLYGLYALAALASERALA
20   TYRVALALASERGLYVALASPLEUTHRGLN
21   ALAALAASNGLUILELYSGLYLEUALAGLN
22   GLNMETGLNLYSLEULEUSERLEUMETLEU
23   GLUHISHISHISHISHISHIS

Entity 2, YopE effector domain 149 residues - 15876.0 Da.

1   METGLUGLYSERHISLYSPROVALVALTHR
2   PROALAPROTHRPROALAGLNMETPROSER
3   PROTHRSERPHESERASPSERILELYSGLN
4   LEUALAALAGLUTHRLEUPROLYSTYRMET
5   GLNGLNLEUASNSERLEUASPALAGLUMET
6   LEUGLNLYSASNHISASPGLNPHEALATHR
7   GLYSERGLYPROLEUARGGLYSERILETHR
8   GLNCYSGLNGLYLEUMETGLNPHECYSGLY
9   GLYGLULEUGLNALAGLUALASERALAILE
10   LEUASNTHRPROVALCYSGLYILEPROPHE
11   SERGLNTRPGLYTHRILEGLYGLYALAALA
12   SERALATYRVALALASERGLYVALASPLEU
13   THRGLNALAALAASNGLUILELYSGLYLEU
14   ALAGLNGLNMETGLNLYSLEULEUSERLEU
15   METLEUGLUHISHISHISHISHISHIS

Samples:

YopE_effector_domain_sample: YopE, [U-95% 15N], 2.8 mM; D2O, [U-99.9% 2H], 5%; H2O 95%; sodium phosphate pH6.1 45 mM

all_samples: pH: 6.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCYopE_effector_domain_sampleisotropicall_samples

Software:

XEASY, Bartels et al., Keller and Wuthrich - chemical shift assignment, chemical shift calculation, data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

BMRB 15878 7426
EMBL CAA68609 CAB54883 CAF25368
GB AAA27672 AAC62587 AAC69818 AAS58592 ABG16295
REF NP_395143 NP_857762 NP_857967 NP_995401 WP_002213405
SP P08008 P31493
AlphaFold P08008 P31493

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks