BMRB Entry 15878

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15878

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Title:
Yersinia pseudotuberculosis type III secretion effector YopE.
Deposition date:
2008-07-17
Original release date:
2008-08-13
Authors:
Rodgers, Loren; Gamez, Alicia; Riek, Roland; Ghosh, Partho
Citation:

Citation: Rodgers, Loren; Gamez, Alicia; Riek, Roland; Ghosh, Partho. "The type III secretion chaperone SycE promotes a localized disorder-to-order transition in the natively unfolded effector YopE."  J. Biol. Chem. 283, 20857-20863 (2008).
PubMed: 18502763

Assembly members:

Assembly members:
YopE, polymer, 227 residues, 24052.1 Da.
SycE, polymer, 130 residues, 14649.6 Da.

Natural source:

Natural source:   Common Name: Yersinia pseudotuberculosis   Taxonomy ID: 633   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Yersinia pseudotuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
YopE: MKISSFISTSLPLPTSVSGS SSVGEMSGRSVSQQTSDQYA NNLAGRTESPQGSSLASRII ERLSSVAHSVIGFIQRMFSE GSHKPVVTPAPTPAQMPSPT SFSDSIKQLAAETLPKYMQQ LNSLDAEMLQKNHAQFATGS GPLRGSITQCQGLMQFCGGE LQAEASAILNTPVCGIPFSQ WGTIGGVASAYVASGVDLTQ AANEIKGLAQQMQKLLSLML EHHHHHH
SycE: MYSFEQAITQLFQQLSLSIP DTIEPVIGVKVGEFACHITE HPVGQILMFTLPSLDNNDEK ETLLSHNIFSQDILKPILSW DEVGGHPVLWNRQPLNSLDN NSLYTQLEMLVQGAERLQTS SLISPPRSFS

Data sets:
Data typeCount
13C chemical shifts216
15N chemical shifts194
1H chemical shifts194

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YopE1
2SycE 12
3SycE 22

Entities:

Entity 1, YopE 227 residues - 24052.1 Da.

1   METLYSILESERSERPHEILESERTHRSER
2   LEUPROLEUPROTHRSERVALSERGLYSER
3   SERSERVALGLYGLUMETSERGLYARGSER
4   VALSERGLNGLNTHRSERASPGLNTYRALA
5   ASNASNLEUALAGLYARGTHRGLUSERPRO
6   GLNGLYSERSERLEUALASERARGILEILE
7   GLUARGLEUSERSERVALALAHISSERVAL
8   ILEGLYPHEILEGLNARGMETPHESERGLU
9   GLYSERHISLYSPROVALVALTHRPROALA
10   PROTHRPROALAGLNMETPROSERPROTHR
11   SERPHESERASPSERILELYSGLNLEUALA
12   ALAGLUTHRLEUPROLYSTYRMETGLNGLN
13   LEUASNSERLEUASPALAGLUMETLEUGLN
14   LYSASNHISALAGLNPHEALATHRGLYSER
15   GLYPROLEUARGGLYSERILETHRGLNCYS
16   GLNGLYLEUMETGLNPHECYSGLYGLYGLU
17   LEUGLNALAGLUALASERALAILELEUASN
18   THRPROVALCYSGLYILEPROPHESERGLN
19   TRPGLYTHRILEGLYGLYVALALASERALA
20   TYRVALALASERGLYVALASPLEUTHRGLN
21   ALAALAASNGLUILELYSGLYLEUALAGLN
22   GLNMETGLNLYSLEULEUSERLEUMETLEU
23   GLUHISHISHISHISHISHIS

Entity 2, SycE 1 130 residues - 14649.6 Da.

1   METTYRSERPHEGLUGLNALAILETHRGLN
2   LEUPHEGLNGLNLEUSERLEUSERILEPRO
3   ASPTHRILEGLUPROVALILEGLYVALLYS
4   VALGLYGLUPHEALACYSHISILETHRGLU
5   HISPROVALGLYGLNILELEUMETPHETHR
6   LEUPROSERLEUASPASNASNASPGLULYS
7   GLUTHRLEULEUSERHISASNILEPHESER
8   GLNASPILELEULYSPROILELEUSERTRP
9   ASPGLUVALGLYGLYHISPROVALLEUTRP
10   ASNARGGLNPROLEUASNSERLEUASPASN
11   ASNSERLEUTYRTHRGLNLEUGLUMETLEU
12   VALGLNGLYALAGLUARGLEUGLNTHRSER
13   SERLEUILESERPROPROARGSERPHESER

Samples:

SycE-YopE: YopE, [U-95% 13C; U-95% 15N; 90% 2H], 0.25 – 1.0 mM; SycE0.5 – 2.0 mM; D2O, [U-99.9% 2H], 5%; H2O 95%; sodium phosphate pH6.1 45 mM

all_samples: pH: 6.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCSycE-YopEisotropicall_samples
3D HNCASycE-YopEisotropicall_samples
3D HNCACBSycE-YopEisotropicall_samples
3D 1H-15N NOESYSycE-YopEisotropicall_samples

Software:

XEASY, Bartels et al., Keller and Wuthrich - chemical shift assignment, chemical shift calculation, data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

EMBL CAA68609 CAB54883 CAF25368 CFU99608 CFV36742 CAB54882 CAF25367 CAL10074 CBW54722 CBY78145
GB AAA27672 AAC62587 AAC69818 AAS58592 ABG16295 AAA27671 AAA27673 AAC62588 AAC69819 AAD16849
REF NP_395143 NP_857762 NP_857967 WP_002229754 WP_002353311 NP_052426 NP_395142 NP_783701 NP_857763 NP_857968
SP P08008 P31493 P31490 P31491
PDB
AlphaFold P08008 P31493 P31490 P31491

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks