BMRB Entry 7391

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7391

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Title:
Electrostatic contributions to the stability of the GCN4 leucine zipper structure.
Deposition date:
2007-06-19
Original release date:
2009-04-15
Authors:
Matousek, William; Alexandrescu, Andrei
Citation:

Citation: Matousek, William; Ciani, Barbara; Fitch, Carolyn; Garcia-Moreno, Bertrand; Kammerer, Richard; Alexandrescu, Andrei. "Electrostatic contributions to the stability of the GCN4 leucine zipper structure."  J. Mol. Biol. 374, 206-219 (2007).
PubMed: 17920624

Assembly members:

Assembly members:
GCN4p-wt, polymer, 33 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: NA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GCN4p-wt: GSMKQLEDKVEELLSKNYHL ENEVARLKKLVGE

Data sets:
Data typeCount
13C chemical shifts94
15N chemical shifts32
1H chemical shifts62

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GCN41

Entities:

Entity 1, GCN4 33 residues - Formula weight is not available

Leucine zipper region of GCN4 yeast transcription activator Met250 Glu280

1   GLYSERMETLYSGLNLEUGLUASPLYSVAL
2   GLUGLULEULEUSERLYSASNTYRHISLEU
3   GLUASNGLUVALALAARGLEULYSLYSLEU
4   VALGLYGLU

Samples:

Denatured_GCN4p-wt_1: GCN4p-wt, [U-13C; U-15N], 1.8 mM; urea 6 M; sodium chloride 150 mM; sodium phosphate 10 mM; D2O 10%

Denatured_GCN4p-wt_cond: pH: 2.3; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBDenatured_GCN4p-wt_1isotropicDenatured_GCN4p-wt_cond
3D HNCADenatured_GCN4p-wt_1isotropicDenatured_GCN4p-wt_cond
3D HN(CO)CADenatured_GCN4p-wt_1isotropicDenatured_GCN4p-wt_cond
3D C(CO)NHDenatured_GCN4p-wt_1isotropicDenatured_GCN4p-wt_cond
3D H(CCO)NHDenatured_GCN4p-wt_1isotropicDenatured_GCN4p-wt_cond
3D HNCODenatured_GCN4p-wt_1isotropicDenatured_GCN4p-wt_cond

Software:

FELIX v2000.1, Accelrys Software Inc. - chemical shift assignment, peak picking, processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 1396 1397 1398 1399 1451 1452 1453 1454 15316 17511 2062 371
PDB
DBJ GAA22815
EMBL CAE52206 CAE52207 CAE52208 CAE52209 CAE52210
GB AAA34640 AAB64486 AAC33186 AAD37724 AAK07887
REF NP_010907
SP P03069
TPG DAA07643
AlphaFold P03069

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks