BMRB Entry 17511

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Leucine Zipper GCN4 coiled coil and x-form conformations.
Published: 2011-04-04

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17511

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Leucine Zipper GCN4 coiled coil and x-form conformations.

Deposition date:

2011-03-06

Original release date:

2011-04-04

Authors:

Nikolaev, Yaroslav; Pervushin, Konstantin

Citation:

Citation: Nikolaev, Yaroslav; Pervushin, Konstantin. "NMR spin state exchange spectroscopy reveals equilibrium of two distinct conformations of leucine zipper GCN4 in solution." J. Am. Chem. Soc. 129, 6461-6469 (2007).
PubMed: 17469817

Assembly members:

Assembly members:
LZ-GCN4, polymer, 36 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LZ-GCN4: GLQRMKQLEDKVEELLSKNY HLENEVARLKKLVGER

Data sets:

assigned_chemical_shifts
- LZ-GCN4_coiled_coil_shifts_at_pH_7.1
- LZ-GCN4_coiled_coil_and_x-form_shifts_at_pH_3.2

Data type	Count
13C chemical shifts	69
15N chemical shifts	104
1H chemical shifts	173

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	LZ-GCN4 dimer, chain 1	1
2	LZ-GCN4 dimer, chain 2	1
3	LZ-GCN4 monomer	1

Entities:

Entity 1, LZ-GCN4 dimer, chain 1 36 residues - Formula weight is not available

First Glycine is a non-native residue from the expression system.

1

GLY

LEU

GLN

ARG

MET

LYS

GLN

LEU

GLU

ASP

2

LYS

VAL

GLU

LEU

SER

LYS

ASN

TYR

3

HIS

LEU

GLU

ASN

GLU

VAL

ALA

ARG

LEU

LYS

4

LYS

LEU

VAL

GLY

GLU

ARG

Samples:

LZ-GCN4_NC: LZ-GCN4, [U-13C; U-15N], 0.3 mM; potassium chloride 80 mM; sodium acetate 40 mM

pH_3.2_310K: pH: 3.2; pressure: 1 atm; temperature: 310 K

pH_7.1_310K: pH: 7.1; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	LZ-GCN4_NC	isotropic	pH_3.2_310K
2D 1H-15N HSQC	LZ-GCN4_NC	isotropic	pH_7.1_310K
3D 1H-15N NOESY	LZ-GCN4_NC	isotropic	pH_7.1_310K

Software:

CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker AMX 600 MHz
Bruker AMX 900 MHz

BMRB	1396 1397 1398 1399 1451 1452 1453 1454 15316 2062 371
PDB	1DGC 1IJ2 1IJ3 1LD4 1LLM 1NKN 1RB4 1RB5 1RB6 1SWI 1YSA 1ZII 1ZIJ 1ZIK 1ZIL 1ZIM 1ZTA 2AHP 2DGC 2ZTA 3BAS 3BAT 3I5C 3K7Z 4DMD 4DME 4NIZ 4NJ0 4TL1
DBJ	GAA22815
EMBL	CAE52206 CAE52207 CAE52208 CAE52209 CAE52210
GB	AAA34640 AAB64486 AAK07887 AAL09032 AHY75543
REF	NP_010907
SP	P03069
TPG	DAA07643
AlphaFold	P03069