BMRB Entry 7320

Title:
PufX structure
Deposition date:
2006-10-20
Original release date:
2006-11-06
Authors:
Tunnicliffe, Richard; Ratcliffe, Emma; Hunter, Neil; Williamson, Mike
Citation:

Citation: Tunnicliffe, Richard; Ratcliffe, Emma; Hunter, Neil; Williamson, Mike. "The solution structure of the PufX polypeptide from Rhodobacter sphaeroides"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
PufX from Rhodobacter sphaeroides, polymer, 82 residues, 9052.6 Da.

Natural source:

Natural source:   Common Name: Rhodobacter sphaeroides   Taxonomy ID: 272943   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Rhodobacter sphaeroides

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PufX from Rhodobacter sphaeroides: MADKTIFNDHLNTNPKTNLR LWVAFQMMKGAGWAGGVFFG TLLLIGFFRVVGRMLPIQEN QAPAPNITGALETGIELIKH LV

Data sets:
Data typeCount
13C chemical shifts299
15N chemical shifts76
1H chemical shifts480

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PufX1

Entities:

Entity 1, PufX 82 residues - 9052.6 Da.

1   METALAASPLYSTHRILEPHEASNASPHIS
2   LEUASNTHRASNPROLYSTHRASNLEUARG
3   LEUTRPVALALAPHEGLNMETMETLYSGLY
4   ALAGLYTRPALAGLYGLYVALPHEPHEGLY
5   THRLEULEULEUILEGLYPHEPHEARGVAL
6   VALGLYARGMETLEUPROILEGLNGLUASN
7   GLNALAPROALAPROASNILETHRGLYALA
8   LEUGLUTHRGLYILEGLULEUILELYSHIS
9   LEUVAL

Samples:

sample_1: PufX from Rhodobacter sphaeroides, [U-15N; U-13C], 1 ± 0.1 mM

conditions_1: temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HNHAsample_1not availableconditions_1
13C/15N-edited_NOESYsample_1not availableconditions_1
TOCSY-HSQCsample_1not availableconditions_1

Software:

FELIX v2004, Accelrys -

NMR spectrometers:

  • Bruker DRX-800 800 MHz
  • Bruker DRX-600 600 MHz

Related Database Links:

BMRB 10024
PDB
EMBL CAB38756
GB AAA26129 AAF24306 ABA79427 ABN77002 ACM01440
REF WP_002720419 YP_001043774 YP_002525941 YP_353328
SP P13402
AlphaFold P13402 P13402

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks