BMRB Entry 10024

Name: Chemical shift assignments for the Rhodobacter sphaeroides PufX membrane protein
Published: 2007-06-13

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PDB ID: 2dw3
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR10024
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shift assignments for the Rhodobacter sphaeroides PufX membrane protein

Deposition date:

2006-08-03

Original release date:

2007-06-13

Authors:

Wang, Zheng-Yu; Suzuki, H.; Kudo, M.; Kobayashi, M.; Nozawa, T.

Citation:

Citation: Wang, Zheng-Yu; Suzuki, H.; Kobayashi, M.; Nozawa, T.. "Solution structure of the Rhodobacter sphaeroides PufX membrane protein: implications for the quinone exchange and protein-protein interactions." Biochemistry 46, 3635-3642 (2007).
PubMed: 17335288

Assembly members:

Assembly members:
PufX polypeptide, polymer, 77 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rhodobacter sphaeroides Taxonomy ID: 1063 Superkingdom: bacteria Kingdom: Not applicable Genus/species: Rhodobacter sphaeroides

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PufX polypeptide: ADKTIFNDHLNTNPKTNLRL WVAFQMMKGAGWAGGVFFGT LLLIGFFRVVGRMLPIQENQ APAPNITGALEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_cs_list_1

Data type	Count
13C chemical shifts	133
15N chemical shifts	74
1H chemical shifts	352

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	subunit 1	1

Entities:

Entity 1, subunit 1 77 residues - Formula weight is not available

1

ALA

ASP

LYS

THR

ILE

PHE

ASN

ASP

HIS

LEU

2

ASN

THR

ASN

PRO

LYS

THR

ASN

LEU

ARG

LEU

3

TRP

VAL

ALA

PHE

GLN

MET

LYS

GLY

ALA

4

GLY

TRP

ALA

GLY

VAL

PHE

GLY

THR

5

LEU

ILE

GLY

PHE

ARG

VAL

6

GLY

ARG

MET

LEU

PRO

ILE

GLN

GLU

ASN

GLN

7

ALA

PRO

ALA

PRO

ASN

ILE

THR

GLY

ALA

LEU

8

GLU

HIS

Samples:

sample_1: PufX polypeptide, [U-13C; U-15N], 2 mM; Chloroform 50%; Methanol 50%

condition_1: temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
HSQC-TOCSY	sample_1	not available	condition_1
HSQC-NOESY	sample_1	not available	condition_1
HNCA	sample_1	not available	condition_1
HN(CO)CA	sample_1	not available	condition_1
CBCANH	sample_1	not available	condition_1
CBCA(CO)NH	sample_1	not available	condition_1
HNHA	sample_1	not available	condition_1

Software:

No software information available

NMR spectrometers:

Bruker Avance DRX400 400 MHz

PDB	2DW3 2ITA 2NRG 4JC9 4JCB
EMBL	CAB38756
GB	AAA26129 AAF24306 ABA79427 ABN77002 ACM01440
REF	WP_002720419 YP_353328
SP	P13402
AlphaFold	P13402