BMRB Entry 7317

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7317

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Title:
Backbone 1HN, 15N, and 13C Chemical Shift Assignments for wt Im7* (* denotes his-tag) and its variants, Im7*L53AI54A and Im7*YY
Deposition date:
2006-10-17
Original release date:
2007-05-04
Authors:
Whittaker, Sara; Spence, Graham; Grossmann, Guenter; Radford, Sheena; Moore, Geoffrey
Citation:

Citation: Whittaker, Sara; Spence, Graham; Grossmann, Guenter; Radford, Sheena; Moore, Geoffrey. "NMR analysis of the conformational properties of the trapped on-pathway folding intermediate of the bacterial immunity protein Im7."  J. Mol. Biol. 366, 1001-1015 (2007).
PubMed: 17188712

Assembly members:

Assembly members:
L53AI54A, polymer, 94 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
L53AI54A: MEHHHHHHELKNSISDYTEA EFVQLLKEIEKENVAATDDV LDVLLEHFVKITEHPDGTDA AYYPSDNRDDSPEGIVKEIK EWRAANGKPGFKQG

Data sets:
Data typeCount
13C chemical shifts195
15N chemical shifts65
1H chemical shifts65

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Monomer1

Entities:

Entity 1, Monomer 94 residues - Formula weight is not available

The sequence of L53AI54A contains an N-terminal His-tag. Residue numbering starts at residue 2 of wt Im7 (no his-tag). Compared with Im7*, the mutations L53A and I54A have been made.

1   METGLUHISHISHISHISHISHISGLULEU
2   LYSASNSERILESERASPTYRTHRGLUALA
3   GLUPHEVALGLNLEULEULYSGLUILEGLU
4   LYSGLUASNVALALAALATHRASPASPVAL
5   LEUASPVALLEULEUGLUHISPHEVALLYS
6   ILETHRGLUHISPROASPGLYTHRASPALA
7   ALATYRTYRPROSERASPASNARGASPASP
8   SERPROGLUGLYILEVALLYSGLUILELYS
9   GLUTRPARGALAALAASNGLYLYSPROGLY
10   PHELYSGLNGLY

Samples:

sample_1: Im7*, [U-13C; U-15N], 1 ± 0.2 mM; potassium phosphate buffer 50 mM; sodium sulphate 400 mM; H2O 90%; D2O 10%

sample_2: Im7*, [U-99% 15N], 1 ± 0.2 mM; potassium phosphate buffer 50 mM; sodium sulphate 400 mM; H2O 90%; D2O 10%

conditions_1: pH: 7; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCnot availablenot availableconditions_1
HNCOnot availablenot availableconditions_1
HNCAnot availablenot availableconditions_1
HNCOCAnot availablenot availableconditions_1
CBCACONHnot availablenot availableconditions_1
HNCACBsample_1not availableconditions_1
1H-1H-15N NOESY-HSQCsample_2not availableconditions_1

Software:

VNMR v6.1C, Varian - Data acquisition

NMRView v5.1.1, Varian - Data analysis

NMR spectrometers:

  • Varian Inova 500 MHz
  • Varian Inova 600 MHz
  • Varian Inova 750 MHz

Related Database Links:

BMRB 7188 7316
PDB
EMBL CAA45165 CDK50318
GB AIC79148 ELE46623 EOW15734 EQR11492 ESD96397
REF WP_001560791 WP_032277812 YP_009060494
SP Q03708
AlphaFold Q03708

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks