BMRB Entry 7188
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7188
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NMR-STAR v3 text file.
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Citation: Le Duff, Cecile; Whittaker, Sara; Radford, Sheena; Moore, Geoffrey. "Characterisation of the Conformational Properties of Urea-unfolded Im7: Implications for the Early Stages of Protein Folding" J. Mol. Biol. 364, 824-835 (2006).
PubMed: 17045607
Assembly members:
Im7 native, polymer, 87 residues, Formula weight is not available
Natural source: Common Name: Escherichia Coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Im7 native: MELKNSISDYTEAEFVQLLK
EIEKENVAATDDVLDVLLEH
FVKITEHPDGTDLIYYPSDN
RDDSPEGIVKEIKEWRAANG
KPGFKQG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 486 |
| 15N chemical shifts | 81 |
| 1H chemical shifts | 153 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Im7 native | 1 |
Entities:
Entity 1, Im7 native 87 residues - Formula weight is not available
| 1 | MET | GLU | LEU | LYS | ASN | SER | ILE | SER | ASP | TYR | ||||
| 2 | THR | GLU | ALA | GLU | PHE | VAL | GLN | LEU | LEU | LYS | ||||
| 3 | GLU | ILE | GLU | LYS | GLU | ASN | VAL | ALA | ALA | THR | ||||
| 4 | ASP | ASP | VAL | LEU | ASP | VAL | LEU | LEU | GLU | HIS | ||||
| 5 | PHE | VAL | LYS | ILE | THR | GLU | HIS | PRO | ASP | GLY | ||||
| 6 | THR | ASP | LEU | ILE | TYR | TYR | PRO | SER | ASP | ASN | ||||
| 7 | ARG | ASP | ASP | SER | PRO | GLU | GLY | ILE | VAL | LYS | ||||
| 8 | GLU | ILE | LYS | GLU | TRP | ARG | ALA | ALA | ASN | GLY | ||||
| 9 | LYS | PRO | GLY | PHE | LYS | GLN | GLY |
Samples:
sample_1: Im7, [U-15N; U-13C], 1 mM; Phosphate buffer 20
sample_2: Im7, [U-15N; U-13C], 1 mM; Phosphate buffer 20 mM; Urea 6 M
conditions_1: pH: 7; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 15N-1H HSQC | not available | not available | conditions_1 |
| 2D 15N-1H HSQC NH2 only | not available | not available | conditions_1 |
| 3D HNCO, 3D HNCA | not available | not available | conditions_1 |
| 3D HNCACB | not available | not available | conditions_1 |
| 3D CBCA(CO)NH | not available | not available | conditions_1 |
| 3D H(CCO)NH | not available | not available | conditions_1 |
| 3D HN(CA)CO | not available | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian Unity Inova 499.865 MHz
Related Database Links:
| BMRB | 7316 7317 |
| PDB | |
| EMBL | CAA45165 CDK50318 |
| GB | AAA23071 AIC79148 ELE46623 EOW15734 EQR11492 |
| REF | WP_001560791 WP_032277812 YP_009060494 |
| SP | Q03708 |
| AlphaFold | Q03708 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks