BMRB Entry 7305
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7305
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Casares, Salvador; AB, Eiso; Eshuis, Henk; Lopez-Mayorga, Obdulio; van Nuland, Nico; Conejero-Lara, Francisco. "The high-resolution NMR structure of the R21A Spc-SH3:P41 complex: Understanding the determinants of binding affinity by comparison with Abl-SH3" BMC Struct. Biol. 7, .-. (2007).
PubMed: 17407569
Assembly members:
R21A Spc-SH3 free, polymer, 62 residues, Formula weight is not available
Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
R21A Spc-SH3 free: MDETGKELVLALYDYQEKSP
AEVTMKKGDILTLLNSTNKD
WWKVEVNDRQGFVPAAYVKK
LD
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 467 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | R21A Spc-SH3 monomer | 1 |
Entities:
Entity 1, R21A Spc-SH3 monomer 62 residues - Formula weight is not available
| 1 | MET | ASP | GLU | THR | GLY | LYS | GLU | LEU | VAL | LEU | ||||
| 2 | ALA | LEU | TYR | ASP | TYR | GLN | GLU | LYS | SER | PRO | ||||
| 3 | ALA | GLU | VAL | THR | MET | LYS | LYS | GLY | ASP | ILE | ||||
| 4 | LEU | THR | LEU | LEU | ASN | SER | THR | ASN | LYS | ASP | ||||
| 5 | TRP | TRP | LYS | VAL | GLU | VAL | ASN | ASP | ARG | GLN | ||||
| 6 | GLY | PHE | VAL | PRO | ALA | ALA | TYR | VAL | LYS | LYS | ||||
| 7 | LEU | ASP |
Samples:
sample_1: R21A Spc-SH3 free 2 mM; H2O 90%; D2O 10%; d5-glycine 20 mM
conditions_1: pH: 3.5; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D TOCSY | sample_1 | not available | conditions_1 |
| 2D NOESY | sample_1 | not available | conditions_1 |
Software:
NMRView v5 -
NMR spectrometers:
- Bruker DRX 750MHz MHz
Related Database Links:
| BMRB | 15144 17915 |
| PDB | |
| DBJ | BAD52438 BAD93097 BAG57892 BAG62120 BAG72795 |
| EMBL | CAA29435 CAA32663 CAA62350 CAB53710 CDQ79062 |
| GB | AAA51702 AAA51790 AAA52468 AAB41498 AAB60364 |
| REF | NP_001036003 NP_001070022 NP_001090674 NP_001091958 NP_001107628 |
| SP | P07751 P16086 P16546 Q13813 |
| TPG | DAA24188 |
| AlphaFold | P07751 P16086 P16546 Q13813 |