BMRB Entry 17915

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PDB ID: 2lj3
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17915
MolProbity Validation Chart

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Title:
PFBD: High-throughput Strategy of Backbone fold Determination for small well-folded proteins in less than a day
Deposition date:
2011-09-04
Original release date:
2011-10-07
Authors:
Kumar, Dinesh; Hosur, Ramakrishna V
Citation:

Citation: Kumar, Dinesh; Borkar, Aditi; Hosur, Ramakrishna. "Facile backbone (1H, 15N, 13Ca, and 13C') assignment of 13C/15N-labeled proteins using orthogonal projection planes of HNN and HN(C)N experiments and its automation."  Magn. Reson. Chem. 50, 357-363 (2012).
PubMed: 22508472

Assembly members:

Assembly members:
SH3, polymer, 62 residues, 7229.313 Da.
LL5, non-polymer, 334.436 Da.

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus Gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX4T1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SH3: MDETGKELVLALYDYQEKSP REVTMKKGDILTLLNSTNKD WWKVEVNDRQGFVPAAYVKK LD

Data sets:
Data typeCount
13C chemical shifts114
15N chemical shifts59
1H chemical shifts59

Additional metadata:

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  • Samples and Experiments
  • Software
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SH31
2LL5_12
3LL5_22
4LL5_32
5LL5_42
6LL5_52

Entities:

Entity 1, SH3 62 residues - 7229.313 Da.

1   METASPGLUTHRGLYLYSGLULEUVALLEU
2   ALALEUTYRASPTYRGLNGLULYSSERPRO
3   ARGGLUVALTHRMETLYSLYSGLYASPILE
4   LEUTHRLEULEUASNSERTHRASNLYSASP
5   TRPTRPLYSVALGLUVALASNASPARGGLN
6   GLYPHEVALPROALAALATYRVALLYSLYS
7   LEUASP

Entity 2, LL5_1 - C16 H22 N4 O2 S - 334.436 Da.

1   LL5

Related Database Links:

BMRB 15013 15144
PDB 1AEY 1BK2 1HD3 1M8M 1NEG 1PWT 1QKW 1QKX 1SHG 1U06 2CDT 2F2V 2F2W 2F2X 2JM8 2JM9 2JMA 2JMC 2LJ3 2NUZ 3M0P 3M0Q 3M0R 3M0S 3M0T 3M0U 3THK 4F16 4F17
DBJ BAD52438 BAD93097 BAG57892 BAG62120 BAG72795
EMBL CAA29435 CAA32663 CAA62350 CAB53710 CAF90367
GB AAA51702 AAA51790 AAA52468 AAB41498 AAB60364
REF NP_001036003 NP_001090674 NP_001091958 NP_001107628 NP_001123910
SP P07751 P16086 P16546 Q13813
TPG DAA24188
AlphaFold P07751 P16086 P16546 Q13813

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks