BMRB Entry 7278

Name: Backbone amide chemical shifts for iG80b E. coli RNase H
Published: 2007-10-09

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR7278

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone amide chemical shifts for iG80b E. coli RNase H

Deposition date:

2006-08-28

Original release date:

2007-10-09

Authors:

Butterwick, Joel; Palmer, Arthur

Citation:

Citation: Butterwick, Joel; Palmer, Arthur. "An inserted Gly residue fine tunes dynamics between mesophilc and thermophilic ribonucleases H" Protein Sci. 15, 2697-2707 (2006).
PubMed: 17088323

Assembly members:

Assembly members:
iG80b ecRNH, polymer, 156 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: Protista Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
iG80b ecRNH: MLKQVEIFTDGSCLGNPGPG GYGAILRYRGREKTFSAGYT RTTNNRMELMAAIVALEALK EHCEVILSTDSQYVRQGITQ GWIHNWKKRGWKTADKKPVK NVDLWQRLDAALGQHQIKWE WVKGHAGHPENERCDELARA AAMNPTLEDTGYQVEV

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
15N chemical shifts	67
1H chemical shifts	67

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	iG80b ecRNH	1

Entities:

Entity 1, iG80b ecRNH 156 residues - Formula weight is not available

1

MET

LEU

LYS

GLN

VAL

GLU

ILE

PHE

THR

ASP

2

GLY

SER

CYS

LEU

GLY

ASN

PRO

GLY

PRO

GLY

3

GLY

TYR

GLY

ALA

ILE

LEU

ARG

TYR

ARG

GLY

4

ARG

GLU

LYS

THR

PHE

SER

ALA

GLY

TYR

THR

5

ARG

THR

ASN

ARG

MET

GLU

LEU

MET

6

ALA

ILE

VAL

ALA

LEU

GLU

ALA

LEU

LYS

7

GLU

HIS

CYS

GLU

VAL

ILE

LEU

SER

THR

ASP

8

SER

GLN

TYR

VAL

ARG

GLN

GLY

ILE

THR

GLN

9

GLY

TRP

ILE

HIS

ASN

TRP

LYS

ARG

GLY

10

TRP

LYS

THR

ALA

ASP

LYS

PRO

VAL

LYS

11

ASN

VAL

ASP

LEU

TRP

GLN

ARG

LEU

ASP

ALA

12

ALA

LEU

GLY

GLN

HIS

GLN

ILE

LYS

TRP

GLU

13

TRP

VAL

LYS

GLY

HIS

ALA

GLY

HIS

PRO

GLU

14

ASN

GLU

ARG

CYS

ASP

GLU

LEU

ALA

ARG

ALA

15

ALA

MET

ASN

PRO

THR

LEU

GLU

ASP

THR

16

GLY

TYR

GLN

VAL

GLU

VAL

Samples:

sample_1: iG80b ecRNH, [U-2H; U-15N], 1.0 ± 0.1 mM

conditions_1: ionic strength: 100 mM; pH: 5.5; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	sample_1	not available	conditions_1
HMQC-NOESY-HSQC	sample_1	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Bruker AV 700 MHz

BMRB	1657 4012
PDB	1F21 1GOA 1GOB 1GOC 1KVA 1KVB 1KVC 1LAV 1LAW 1RBR 1RBS 1RBT 1RBU 1RBV 1RCH 1RDA 1RDB 1RDC 1RDD 1RNH 1WSE 1WSF 1WSG 1WSH 1WSI 1WSJ 2RN2 3AA2 3AA3 3AA4 3AA5
DBJ	BAA77885 BAB33633 BAG75734 BAI23570 BAI29084
EMBL	CAA23620 CAA27660 CAP74778 CAQ30729 CAQ87812
GB	AAA24565 AAB08636 AAC73319 AAG54510 AAN41862
REF	NP_285902 NP_308237 NP_414750 NP_706155 NP_752197
SP	A7ZHV1 A7ZWF6 B1IPU4 B1LHM3 B1XD78
AlphaFold	A7ZHV1 A7ZWF6 B1IPU4 B1LHM3 B1XD78