BMRB Entry 4012

Name: Structure of the Acid State of E. coli Ribonuclease HI
Published: 2001-02-16

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4012

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of the Acid State of E. coli Ribonuclease HI

Deposition date:

1996-06-21

Original release date:

2001-02-16

Authors:

Dabora, Jonathan; Pelton, Jeffrey; Marqusee, Susan

Citation:

Citation: Dabora, Jonathan; Pelton, Jeffrey; Marqusee, Susan. "Structure of the Acid State of E. coli Ribonuclease H1" Biochemistry 35, 11951-11958 (1996).
PubMed: 8810899

Assembly members:

Assembly members:
Ribonuclease H1, polymer, 155 residues, 17502 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSM101

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ribonuclease H1: MLKQVEIFTDGSALGNPGPG GYGAILRYRGREKTFSAGYT RTTNNRMELMAAIVALEALK EHAEVILSTDSQYVRQGITQ WIHNWKKRGWKTADKKPVKN VDLWQRLDAALGQHQIKWEW VKGHAGHPENERADELARAA AMNPTLEDTGYQVEV

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_one

Data type	Count
1H chemical shifts	276
13C chemical shifts	138
15N chemical shifts	138

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RNase H1	1

Entities:

Entity 1, RNase H1 155 residues - 17502 Da.

1

MET

LEU

LYS

GLN

VAL

GLU

ILE

PHE

THR

ASP

2

GLY

SER

ALA

LEU

GLY

ASN

PRO

GLY

PRO

GLY

3

GLY

TYR

GLY

ALA

ILE

LEU

ARG

TYR

ARG

GLY

4

ARG

GLU

LYS

THR

PHE

SER

ALA

GLY

TYR

THR

5

ARG

THR

ASN

ARG

MET

GLU

LEU

MET

6

ALA

ILE

VAL

ALA

LEU

GLU

ALA

LEU

LYS

7

GLU

HIS

ALA

GLU

VAL

ILE

LEU

SER

THR

ASP

8

SER

GLN

TYR

VAL

ARG

GLN

GLY

ILE

THR

GLN

9

TRP

ILE

HIS

ASN

TRP

LYS

ARG

GLY

TRP

10

LYS

THR

ALA

ASP

LYS

PRO

VAL

LYS

ASN

11

VAL

ASP

LEU

TRP

GLN

ARG

LEU

ASP

ALA

12

LEU

GLY

GLN

HIS

GLN

ILE

LYS

TRP

GLU

TRP

13

VAL

LYS

GLY

HIS

ALA

GLY

HIS

PRO

GLU

ASN

14

GLU

ARG

ALA

ASP

GLU

LEU

ALA

ARG

ALA

15

ALA

MET

ASN

PRO

THR

LEU

GLU

ASP

THR

GLY

16

TYR

GLN

VAL

GLU

VAL

Samples:

sample_one: Ribonuclease H1, [U-99% 13C; U-99% 15N], 1 – 3 mM

sample_conditions_one: pH: 5.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

No software information available

NMR spectrometers:

Bruker AMX 600 MHz

BMRB	1657
PDB	1F21 1G15 1GOA 1GOB 1JL1 1JXB 1KVA 1KVB 1KVC 1LAV 1LAW 1RBR 1RBS 1RBT 1RBU 1RBV 1RCH 1RDA 1RDB 1RDC 1RDD 2RN2 3AA2 3AA3 3AA4 3AA5 4Z0U
DBJ	BAA77885 BAB33633 BAG75734 BAI23570 BAI29084
EMBL	CAA23620 CAA27660 CAP74778 CAQ30729 CAQ87812
GB	AAA24565 AAB08636 AAC73319 AAG54510 AAN41862
REF	NP_308237 NP_414750 NP_706155 WP_000917867 WP_000917875
SP	A7ZHV1 A7ZWF6 B1IPU4 B1LHM3 B1XD78
AlphaFold	A7ZHV1 A7ZWF6 B1IPU4 B1LHM3 B1XD78