BMRB Entry 7277

Name: Backbone amide chemical shifts for dG85 T. thermophilus RNase H
Published: 2007-10-09

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7277

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone amide chemical shifts for dG85 T. thermophilus RNase H

Deposition date:

2006-08-28

Original release date:

2007-10-09

Authors:

Butterwick, Joel; Palmer, Arthur

Citation:

Citation: Butterwick, Joel; Palmer, Arthur. "An inserted Gly residue fine tunes dynamics between mesophilic and thermophilic ribonucleases H" Protein Sci. 15, 2697-2707 (2006).
PubMed: 17088323

Assembly members:

Assembly members:
dG85 ttRNH, polymer, 165 residues, Formula weight is not available

Natural source:

Natural source: Common Name: T. thermophilus Taxonomy ID: 274 Superkingdom: Eubacteria Kingdom: Protista Genus/species: Thermus thermophilus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dG85 ttRNH: MNPSPRKRVALFTDGAALGN PGPGGWAALLRFHAHEKLLS GGEASTTNNRMELKAAIEGL KALKEPAEVDLYTDSHYLKK AFTEWLEGWRKRGWRTAEGK PVKNRDLWEALLLAMAPHRV RFHFVKGHTGHPENERVDRE ARRQAQSQAKTPSPPRAPTL FHEEA

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
15N chemical shifts	144
1H chemical shifts	144

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	dG85 ttRNH	1

Entities:

Entity 1, dG85 ttRNH 165 residues - Formula weight is not available

1

MET

ASN

PRO

SER

PRO

ARG

LYS

ARG

VAL

ALA

2

LEU

PHE

THR

ASP

GLY

ALA

LEU

GLY

ASN

3

PRO

GLY

PRO

GLY

TRP

ALA

LEU

4

ARG

PHE

HIS

ALA

HIS

GLU

LYS

LEU

SER

5

GLY

GLU

ALA

SER

THR

ASN

ARG

6

MET

GLU

LEU

LYS

ALA

ILE

GLU

GLY

LEU

7

LYS

ALA

LEU

LYS

GLU

PRO

ALA

GLU

VAL

ASP

8

LEU

TYR

THR

ASP

SER

HIS

TYR

LEU

LYS

9

ALA

PHE

THR

GLU

TRP

LEU

GLU

GLY

TRP

ARG

10

LYS

ARG

GLY

TRP

ARG

THR

ALA

GLU

GLY

LYS

11

PRO

VAL

LYS

ASN

ARG

ASP

LEU

TRP

GLU

ALA

12

LEU

ALA

MET

ALA

PRO

HIS

ARG

VAL

13

ARG

PHE

HIS

PHE

VAL

LYS

GLY

HIS

THR

GLY

14

HIS

PRO

GLU

ASN

GLU

ARG

VAL

ASP

ARG

GLU

15

ALA

ARG

GLN

ALA

GLN

SER

GLN

ALA

LYS

16

THR

PRO

SER

PRO

ARG

ALA

PRO

THR

LEU

17

PHE

HIS

GLU

ALA

Samples:

sample_1: dG85 ttRNH, [U-2H; U-15N], 0.3 mM; sodium acetate-d3 100 mM; DSS 1 mM; NaN3 0.02 %(w/v); D2O 10%; H2O 90%

conditions_1: ionic strength: 100 mM; pH: 5.5; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	sample_1	not available	conditions_1
HMQC-NOESY-HSQC	sample_1	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Bruker AV 700 MHz

BMRB	5918
PDB	1RIL
DBJ	BAD71379
EMBL	CAA43026
PIR	A42673
REF	WP_011228761 YP_144822
SP	P29253
AlphaFold	P29253