BMRB Entry 5918

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5918

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Multiple time-scale dynamics of homologous thermophilic and mesophilic ribonuclease HI enzymes

Deposition date:

2003-08-21

Original release date:

2004-07-23

Authors:

Butterwick, Joel; Patrick, Loria; Astrof, Nathan; Kroenke, Christopher; Cole, Roger; Rance, Mark; Palmer, Arthur

Citation:

Citation: Butterwick, Joel; Patrick, Loria; Astrof, Nathan; Kroenke, Christopher; Cole, Roger; Rance, Mark; Palmer, Arthur. "Multiple time-scale backbone dynamics of homologous thermophilic and mesophilic ribonuclease HI enzymes" J. Mol. Biol. 339, 855-871 (2004).
PubMed: 15165855

Assembly members:

Assembly members:
Ribonuclease HI, polymer, 166 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Thermus thermophilus Taxonomy ID: 274 Superkingdom: Eubacteria Kingdom: not available Genus/species: Thermus thermophilus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ribonuclease HI: MNPSPRKRVALFTDGAALGN PGPGGWAALLRFHAHEKLLS GGEASTTNNRMELKAAIEGL KALKEPAEVDLYTDSHYLKK AFTEGWLEGWRKRGWRTAEG KPVKNRDLWEALLLAMAPHR VRFHFVKGHTGHPENERVDR EARRQAQSQAKTPSPPRAPT LFHEEA

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	439
15N chemical shifts	149
1H chemical shifts	149

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ttRNH monomer	1

Entities:

Entity 1, ttRNH monomer 166 residues - Formula weight is not available

1

MET

ASN

PRO

SER

PRO

ARG

LYS

ARG

VAL

ALA

2

LEU

PHE

THR

ASP

GLY

ALA

LEU

GLY

ASN

3

PRO

GLY

PRO

GLY

TRP

ALA

LEU

4

ARG

PHE

HIS

ALA

HIS

GLU

LYS

LEU

SER

5

GLY

GLU

ALA

SER

THR

ASN

ARG

6

MET

GLU

LEU

LYS

ALA

ILE

GLU

GLY

LEU

7

LYS

ALA

LEU

LYS

GLU

PRO

ALA

GLU

VAL

ASP

8

LEU

TYR

THR

ASP

SER

HIS

TYR

LEU

LYS

9

ALA

PHE

THR

GLU

GLY

TRP

LEU

GLU

GLY

TRP

10

ARG

LYS

ARG

GLY

TRP

ARG

THR

ALA

GLU

GLY

11

LYS

PRO

VAL

LYS

ASN

ARG

ASP

LEU

TRP

GLU

12

ALA

LEU

ALA

MET

ALA

PRO

HIS

ARG

13

VAL

ARG

PHE

HIS

PHE

VAL

LYS

GLY

HIS

THR

14

GLY

HIS

PRO

GLU

ASN

GLU

ARG

VAL

ASP

ARG

15

GLU

ALA

ARG

GLN

ALA

GLN

SER

GLN

ALA

16

LYS

THR

PRO

SER

PRO

ARG

ALA

PRO

THR

17

LEU

PHE

HIS

GLU

ALA

Samples:

sample_1: Ribonuclease HI, [U-90% 2H; U-96% 13C; U-96% 15N], 0.3 mM; sodium acetate-d3 100 mM; sodium azide 0.02%; D2O 10%

sample_conditions_1: pH: 5.5 na; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
[1H,15N]-TROSY Triple-Resonance	not available	not available	not available
HNCA	not available	not available	not available
HN(CO)CA	not available	not available	not available
HN(COCA)CB	not available	not available	not available
HNCO	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Bruker DRX 600 MHz

BMRB	7277
PDB	1RIL
DBJ	BAD71379
EMBL	CAA43026
PIR	A42673
REF	WP_011228761 YP_144822
SP	P29253
AlphaFold	P29253