BMRB Entry 7222

Name: Amide chemical shifts of free and hyaluronan-bound Link_TSG6 at a range of pH values
Published: 2007-03-06

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR7222

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Amide chemical shifts of free and hyaluronan-bound Link_TSG6 at a range of pH values

Deposition date:

2006-07-12

Original release date:

2007-03-06

Authors:

Blundell, Charles; Day, Anthony

Citation:

Citation: Blundell, Charles; Mahoney, David; Cordell, Martin; Almond, Andrew; Kahmann, Jan; Perczel, Andras; Taylor, Jon; Campbell, Iain; Day, Anthony. "Determining the molecular basis for the pH-dependent interaction between the Link module of human TSG-6 and hyaluronan" J. Biol. Chem. 282, 12976-12988 (2007).
PubMed: 17307731

Assembly members:

Assembly members:
Link_TSG6, polymer, 98 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Link_TSG6: GVYHREARSGKYKLTYAEAK AVCEFEGGHLATYKQLEAAR KIGFHVCAAGWMAKGRVGYP IVKPGPNCGFGKTGIIDYGI RLNRSERWDAYCYNPHAK

Data sets:

assigned_chemical_shifts

Data type	Count
15N chemical shifts	1080
1H chemical shifts	1412

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Link_TSG6	1

Entities:

Entity 1, Link_TSG6 98 residues - Formula weight is not available

1

GLY

VAL

TYR

HIS

ARG

GLU

ALA

ARG

SER

GLY

2

LYS

TYR

LYS

LEU

THR

TYR

ALA

GLU

ALA

LYS

3

ALA

VAL

CYS

GLU

PHE

GLU

GLY

HIS

LEU

4

ALA

THR

TYR

LYS

GLN

LEU

GLU

ALA

ARG

5

LYS

ILE

GLY

PHE

HIS

VAL

CYS

ALA

GLY

6

TRP

MET

ALA

LYS

GLY

ARG

VAL

GLY

TYR

PRO

7

ILE

VAL

LYS

PRO

GLY

PRO

ASN

CYS

GLY

PHE

8

GLY

LYS

THR

GLY

ILE

ASP

TYR

GLY

ILE

9

ARG

LEU

ASN

ARG

SER

GLU

ARG

TRP

ASP

ALA

10

TYR

CYS

TYR

ASN

PRO

HIS

ALA

LYS

Samples:

sample_1: Link_TSG6, [U-15N], 1 ± 0.1 mM; H2O 90%; D2O 10%; sodium azide 0.02 %(w/v)

conditions_1: pH: 4.75; temperature: 298 K

conditions_2: pH: 5.0; temperature: 298 K

conditions_3: pH: 5.25; temperature: 298 K

conditions_4: pH: 5.5; temperature: 298 K

conditions_5: pH: 5.75; temperature: 298 K

conditions_6: pH: 6.0; temperature: 298 K

conditions_7: pH: 6.25; temperature: 298 K

conditions_8: pH: 6.5; temperature: 298 K

conditions_9: pH: 6.75; temperature: 298 K

conditions_10: pH: 7.0; temperature: 298 K

conditions_11: pH: 7.25; temperature: 298 K

conditions_12: pH: 7.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N_HSQC_(example)	sample_1	not available	not available

Software:

No software information available

NMR spectrometers:

GE Omega 500 MHz

BMRB	6392 6393 7221
PDB	1O7B 1O7C 2PF5
DBJ	BAE24204 BAJ20799
EMBL	CAD12353 CAD13434 CAH74219
GB	AAA03342 AAB00792 AAC53527 AAH21155 AAH30205
REF	NP_001007814 NP_001075375 NP_001075780 NP_001252869 NP_009046
SP	O08859 P98065 P98066
TPG	DAA32671
AlphaFold	O08859 P98065 P98066