BMRB Entry 6393

Name: Refined solution structure of the human TSG-6 Link module
Published: 2005-04-12

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PDB ID: 1o7c
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6393
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Refined solution structure of the human TSG-6 Link module

Deposition date:

2004-11-17

Original release date:

2005-04-12

Authors:

Blundell, C.; Teriete, P.; Kahmann, J.; Pickford, A.; Campbell, I.

Citation:

Citation: Blundell, C.; Almond, A.; Mahoney, D.; Deangelis, P.; Campbell, I.; Day, A.. "Towards a structure for a hyaluronan-TSG-6 complex by modeling and NMR spectroscopy: Insights into other members of the link module superfamily" J. Biol. Chem. ., .-..
PubMed: 15718240

Assembly members:

Assembly members:
human TSG-6, polymer, 98 residues, Formula weight is not available
HA octasaccharide, polymer, 8 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human TSG-6: GVYHREARSGKYKLTYAEAK AVCEFEGGHLATYKQLEAAR KIGFHVCAAGWMAKGRVGYP IVKPGPNCGFGKTGIIDYGI RLNRSERWDAYCYNPHAK
HA octasaccharide: XXXXXXXX

Data sets:

assigned_chemical_shifts
- chem_shift_set_1

Data type	Count
1H chemical shifts	681
13C chemical shifts	427
15N chemical shifts	113

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Link TSG6	1
2	HA octasaccharide	2

Entities:

Entity 1, Link TSG6 98 residues - Formula weight is not available

1

GLY

VAL

TYR

HIS

ARG

GLU

ALA

ARG

SER

GLY

2

LYS

TYR

LYS

LEU

THR

TYR

ALA

GLU

ALA

LYS

3

ALA

VAL

CYS

GLU

PHE

GLU

GLY

HIS

LEU

4

ALA

THR

TYR

LYS

GLN

LEU

GLU

ALA

ARG

5

LYS

ILE

GLY

PHE

HIS

VAL

CYS

ALA

GLY

6

TRP

MET

ALA

LYS

GLY

ARG

VAL

GLY

TYR

PRO

7

ILE

VAL

LYS

PRO

GLY

PRO

ASN

CYS

GLY

PHE

8

GLY

LYS

THR

GLY

ILE

ASP

TYR

GLY

ILE

9

ARG

LEU

ASN

ARG

SER

GLU

ARG

TRP

ASP

ALA

10

TYR

CYS

TYR

ASN

PRO

HIS

ALA

LYS

Entity 2, HA octasaccharide 8 residues - Formula weight is not available

1

BNA

BGC

BNA

BGC

BNA

BGC

BNA

BGC

Samples:

sample_1: human TSG-6 1.0 mM; HA octasaccharide 1.0 mM; Na ions 2 mM; D2O 100%

sample_2: human TSG-6, [U-15N], 2.0 mM; HA octasaccharide 1.0 mM; Na ions 2 mM

sample_3: human TSG-6, [U-15N; U-13C], 2.8 mM; HA octasaccharide 1.0 mM; Na ions 2 mM

sample_cond_1: pH: 6.0; temperature: 298 K; ionic strength: 2 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
15N-NOESY-HSQC	not available	not available	not available
15N-HSQC-NOESY-HSQC	not available	not available	not available
13C-NOESY-HSQC	not available	not available	not available
2D-NOESY	not available	not available	not available

Software:

FELIX v2.3 - spectrum processing

XEasy - spectrum analysis

NMR spectrometers:

n/a n/a 750 MHz
n/a n/a 750 MHz

BMRB	6392 7221 7222
PDB	1O7B 1O7C 2PF5
DBJ	BAE24204 BAJ20799
EMBL	CAD12353 CAD13434 CAH74219
GB	AAA03342 AAB00792 AAC53527 AAH21155 AAH30205
REF	NP_001007814 NP_001075375 NP_001075780 NP_001252869 NP_009046
SP	O08859 P98065 P98066
TPG	DAA32671
AlphaFold	O08859 P98065 P98066