BMRB Entry 7141

Name: SH2 domain of Human Csk, complex with Cbp
Published: 2011-05-26

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7141

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SH2 domain of Human Csk, complex with Cbp

Deposition date:

2006-06-02

Original release date:

2011-05-26

Authors:

Liu, Dongsheng; Cowburn, David

Citation:

Citation: Liu, Dongsheng; Cowburn, David. "Domain interactions in protein tyrosine kinase Csk identified by segmental labeling NMR methods" Proc. Natl. Acad. Sci. U.S.A. ., .-..

Assembly members:

Assembly members:
SH2, polymer, 106 residues, 425.883 Da.
2-{4-[4-(4-CHLORO-PHENOXY)-BENZENESULFONYL]-TETRAHYDRO-PYRAN-4-YL}-N-HYDROXY-ACETAMIDE, non-polymer, 425.883 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SH2: AGTKLSLMPWFHGKITREQA ERLLYPPETGLFLVRESTNY PGDYTLCVSCDGKVEHYRIM YHASKLSIDEEVYFENLMQL VEHYTSDADGLCTRLIKPKV MEGTVA

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	196
15N chemical shifts	96
1H chemical shifts	96

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SH2 of Csk	1
2	Cbp	2

Entities:

Entity 1, SH2 of Csk 106 residues - 425.883 Da.

1

ALA

GLY

THR

LYS

LEU

SER

LEU

MET

PRO

TRP

2

PHE

HIS

GLY

LYS

ILE

THR

ARG

GLU

GLN

ALA

3

GLU

ARG

LEU

TYR

PRO

GLU

THR

GLY

4

LEU

PHE

LEU

VAL

ARG

GLU

SER

THR

ASN

TYR

5

PRO

GLY

ASP

TYR

THR

LEU

CYS

VAL

SER

CYS

6

ASP

GLY

LYS

VAL

GLU

HIS

TYR

ARG

ILE

MET

7

TYR

HIS

ALA

SER

LYS

LEU

SER

ILE

ASP

GLU

8

GLU

VAL

TYR

PHE

GLU

ASN

LEU

MET

GLN

LEU

9

VAL

GLU

HIS

TYR

THR

SER

ASP

ALA

ASP

GLY

10

LEU

CYS

THR

ARG

LEU

ILE

LYS

PRO

LYS

VAL

11

MET

GLU

GLY

THR

VAL

ALA

Entity 2, Cbp - C19 H20 Cl N O6 S - 425.883 Da.

1

CBP

Samples:

sample_1: SH2 0.8 ± 0.1 mM; CBP

conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N_HSQC_(example)	sample_1	not available	conditions_1
HNCA	sample_1	not available	conditions_1
HNCACB	sample_1	not available	conditions_1
CBCA(CO)NH	sample_1	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Bruker DRX 800 MHz

BMRB	7140
PDB	1K9A 3EAC 3EAZ
DBJ	BAD97346 BAE23297 BAE33565 BAE42047 BAF83220
EMBL	CAA41484 CAA42556 CAA42713 CAB58562 CAG46758
GB	AAA18766 AAH18394 AAH52006 AAH98863 AAI04848
PRF	1709363A
REF	NP_001025210 NP_001068865 NP_001120662 NP_001177102 NP_001248565
SP	P32577 P41240 P41241 Q0VBZ0
TPG	DAA17512
AlphaFold	P32577 P41240 P41241 Q0VBZ0