BMRB Entry 7140
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7140
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NMR-STAR v3 text file.
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Citation: Cowburn, David; Liu, Dongsheng. "Domain interactions in protein tyrosine kinase Csk identified by segmental labeling NMR methods" Proc. Natl. Acad. Sci. U.S.A. ., .-..
Assembly members:
SH2, polymer, 106 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
SH2: AGTKLSLMPWFHGKITREQA
ERLLYPPETGLFLVRESTNY
PGDYTLCVSCDGKVEHYRIM
YHASKLSIDEEVYFENLMQL
VEHYTSDADGLCTRLIKPKV
MEGTVA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 179 |
| 15N chemical shifts | 92 |
| 1H chemical shifts | 92 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH2 domain of human Csk | 1 |
Entities:
Entity 1, SH2 domain of human Csk 106 residues - Formula weight is not available
| 1 | ALA | GLY | THR | LYS | LEU | SER | LEU | MET | PRO | TRP | ||||
| 2 | PHE | HIS | GLY | LYS | ILE | THR | ARG | GLU | GLN | ALA | ||||
| 3 | GLU | ARG | LEU | LEU | TYR | PRO | PRO | GLU | THR | GLY | ||||
| 4 | LEU | PHE | LEU | VAL | ARG | GLU | SER | THR | ASN | TYR | ||||
| 5 | PRO | GLY | ASP | TYR | THR | LEU | CYS | VAL | SER | CYS | ||||
| 6 | ASP | GLY | LYS | VAL | GLU | HIS | TYR | ARG | ILE | MET | ||||
| 7 | TYR | HIS | ALA | SER | LYS | LEU | SER | ILE | ASP | GLU | ||||
| 8 | GLU | VAL | TYR | PHE | GLU | ASN | LEU | MET | GLN | LEU | ||||
| 9 | VAL | GLU | HIS | TYR | THR | SER | ASP | ALA | ASP | GLY | ||||
| 10 | LEU | CYS | THR | ARG | LEU | ILE | LYS | PRO | LYS | VAL | ||||
| 11 | MET | GLU | GLY | THR | VAL | ALA |
Samples:
sample_1: SH2, [U-95% 13C; U-90% 15N], 0.8 ± 0.1 mM
conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC_(example) | sample_1 | not available | conditions_1 |
| HNCA | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| CBCA(CO)NH | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 800 MHz
Related Database Links:
| BMRB | 7141 |
| PDB | |
| DBJ | BAD97346 BAE23297 BAE33565 BAE42047 BAF83220 |
| EMBL | CAA41484 CAA42556 CAA42713 CAB58562 CAG46758 |
| GB | AAA18766 AAH18394 AAH52006 AAH98863 AAI04848 |
| PRF | 1709363A |
| REF | NP_001025210 NP_001068865 NP_001120662 NP_001177102 NP_001248565 |
| SP | P32577 P41240 P41241 Q0VBZ0 |
| TPG | DAA17512 |
| AlphaFold | P32577 P41240 P41241 Q0VBZ0 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks