BMRB Entry 7089

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PDB ID: 2mls
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7089
MolProbity Validation Chart

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Title:
1H, 13C, and 15N Peak Assignments of Human Macrophage Metalloelastase, in its inhibitor-free state
Deposition date:
2006-04-26
Original release date:
2007-05-02
Authors:
Bhaskaran, Rajagopalan; VanDoren, Steven
Citation:

Citation: Bhaskaran, Rajagopalan; VanDoren, Steven. "1H, 13C, and 15N peak assignments and secondary structure of human macrophage metalloelastase (MMP-12) in its inhibitor-free state"  J. Biomol. NMR 36, 55-55 (2006).
PubMed: 16855860

Assembly members:

Assembly members:
MMP-12, polymer, 164 residues, 18152.7 Da.
ZN, non-polymer, 65.409 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pGEXMEX-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MMP-12: FREMPGGPVWRKHYITYRIN NYTPDMNREDVDYAIRKAFQ VWSNVTPLKFSKINTGMADI LVVFARGAHGDFHAFDGKGG ILAHAFGPGSGIGGDAHFDE DEFWTTHSGGTNLFLTAVHA IGHSLGLGHSSDPKAVMFPT YKYVDINTFRLSADDIRGIQ SLYG

Data sets:
Data typeCount
13C chemical shifts638
15N chemical shifts166
1H chemical shifts958

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Metalloprotease1
2ZINC (II) ION, 12
3ZINC (II) ION, 22
4CALCIUM (II) ION, 13
5CALCIUM (II) ION, 23
6CALCIUM (II) ION, 33

Entities:

Entity 1, Metalloprotease 164 residues - 18152.7 Da.

The residue's author sequnce code corresponds to the original publication of the full-length protein sequence

1   PHEARGGLUMETPROGLYGLYPROVALTRP
2   ARGLYSHISTYRILETHRTYRARGILEASN
3   ASNTYRTHRPROASPMETASNARGGLUASP
4   VALASPTYRALAILEARGLYSALAPHEGLN
5   VALTRPSERASNVALTHRPROLEULYSPHE
6   SERLYSILEASNTHRGLYMETALAASPILE
7   LEUVALVALPHEALAARGGLYALAHISGLY
8   ASPPHEHISALAPHEASPGLYLYSGLYGLY
9   ILELEUALAHISALAPHEGLYPROGLYSER
10   GLYILEGLYGLYASPALAHISPHEASPGLU
11   ASPGLUPHETRPTHRTHRHISSERGLYGLY
12   THRASNLEUPHELEUTHRALAVALHISALA
13   ILEGLYHISSERLEUGLYLEUGLYHISSER
14   SERASPPROLYSALAVALMETPHEPROTHR
15   TYRLYSTYRVALASPILEASNTHRPHEARG
16   LEUSERALAASPASPILEARGGLYILEGLN
17   SERLEUTYRGLY

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1   ZN

Entity 3, CALCIUM (II) ION, 1 - Ca - 40.078 Da.

1   CA

Related Database Links:

BMRB 15578 6391 6444 7415
PDB 1JIZ 1JK3 1OS2 1OS9 1RMZ 1ROS 1UTT 1UTZ 1Y93 1YCM 1Z3J 2HU6 2K2G 2K9C 2KRJ 2MLR 2MLS 2OXU 2OXW 2OXZ 2POJ 2W0D 2WO8 2WO9 2WOA 2Z2D 3BA0 3EHX 3EHY 3F15 3F16 3F17 3F18 3F19 3F1A 3LIK 3LIL 3LIR 3LJG 3LK8 3LKA 3N2U 3N2V 3NX7 3RTS 3RTT 3TS4 3TSK 3UVC 4EFS 4GQL 4GR0 4GR3 4GR8 4GUY 4H30 4H49 4H76 4H84 4I03 4IJO
DBJ BAG36675 BAJ20684
GB AAA58658 AAI12302 AAI43774 AAW29944 ADR83017
REF NP_002417 XP_003828422 XP_004052087 XP_508724
SP P39900
AlphaFold P39900

Download HSQC peak lists in one of the following formats:
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