BMRB Entry 6391

Title:
1H, 13C, and 15N chemical shift assignments for the catalytic domain of MMP-12
Deposition date:
2004-11-16
Original release date:
2005-04-08
Authors:
Markus, Michelle; Dwyer, Brian; Wolfrom, Scott; Li, Jianchang; Li, Wei; Malakian, Karl; Wilhelm, James; Tsao, Desiree
Citation:

Citation: Markus, Michelle; Dwyer, Brian; Wolfrom, Scott; Li, Jianchang; Li, Wei; Malakian, Karl; Wilhelm, James; Tsao, Desiree. "1H, 13C, and 15N assignments of MMP-12, a key protease implicated in lung tissue remodeling"  J. Biomol. NMR 31, 260-260 (2005).

Assembly members:

Assembly members:
mmp12, polymer, 165 residues, 18342.6 Da.
DSV, non-polymer, 363.451 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts663
15N chemical shifts169
1H chemical shifts980

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mmp121
2inhibitor2

Entities:

Entity 1, mmp12 165 residues - 18342.6 Da.

1   METPHEARGGLUMETPROGLYGLYPROVAL
2   TRPARGLYSHISTYRILETHRTYRARGILE
3   ASNASNTYRTHRPROASPMETASNARGGLU
4   ASPVALASPTYRALAILEARGLYSALAPHE
5   GLNVALTRPSERASNVALTHRPROLEULYS
6   PHESERLYSILEASNTHRGLYMETALAASP
7   ILELEUVALVALPHEALAARGGLYALAHIS
8   GLYASPPHEHISALAPHEASPGLYLYSGLY
9   GLYILELEUALAHISALAPHEGLYPROGLY
10   SERGLYILEGLYGLYASPALAHISPHEASP
11   GLUASPGLUPHETRPTHRTHRHISSERGLY
12   GLYTHRASNLEUPHELEUTHRALAVALHIS
13   GLUILEGLYHISSERLEUGLYLEUGLYHIS
14   SERSERASPPROLYSALAVALMETPHEPRO
15   THRTYRLYSTYRVALASPILEASNTHRPHE
16   ARGLEUSERALAASPASPILEARGGLYILE
17   GLNSERLEUTYRGLY

Entity 2, inhibitor - C17 H17 N O4 S2 - 363.451 Da.

1   DSV

Samples:

15N_sample: mmp12, [U-15N], 0.2 mM; inhibitor 0.2 mM

13C15N_sample: mmp12, [U-13C; U-15N], 0.7 mM; inhibitor 0.7 mM

13C15N_D2O_sample: mmp12, [U-13C; U-15N], 0.4 mM; inhibitor 0.4 mM; D2O 100%

10percent_13C_sample: mmp12, [U-10% 13C], 0.55 mM; inhibitor 0.55 mM

conditions: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availableconditions
HNCAnot availablenot availableconditions
HNCACBnot availablenot availableconditions
CBCA(CO)NHnot availablenot availableconditions
HN(CA)COnot availablenot availableconditions
C(CO)NHnot availablenot availableconditions
H(CCO)NHnot availablenot availableconditions
HBHA(CO)NHnot availablenot availableconditions
HCCH TOCSYnot availablenot availableconditions
HACAHBnot availablenot availableconditions
HNHBnot availablenot availableconditions
1H13C HSQCnot availablenot availableconditions

Software:

PIPP v4.2.2, Dan Garrett - peak picking

NMRPipe v2.3, Frank Delaglio - processing spectra

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 15578 6444 7089 7415
PDB
DBJ BAG36675 BAJ20684
GB AAA58658 AAI12302 AAI43774 AAW29944 ADR83017
REF NP_002417 XP_003828422 XP_004052087 XP_508724
SP P39900
AlphaFold P39900

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks