BMRB Entry 6942

Name: 1H, 13C, and 15N Chemical Shift Assignments for Myristoylated neuronal calcium sensor-1
Published: 2006-08-07

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6942

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N Chemical Shift Assignments for Myristoylated neuronal calcium sensor-1

Deposition date:

2006-01-18

Original release date:

2006-08-07

Authors:

Mukherjee, Sulakshana; Chary, Kandala

Citation:

Citation: Mukherjee, Sulakshana; Muralidhar, Dasari; Atreya, Hanudatta; Szyperski, Thomas; Jeromin, Andreas; Sharma, Yogendra; Chary, Kandala. "1H, 13C, and 15N chemical shift assignments of neuronal calcium sensor-1, a multi-functional calcium-binding protein" J. Biomol. NMR 36, 48-48 (2006).
PubMed: 16804767

Assembly members:

Assembly members:
myristoylated neuronal calcium sensor-1 protein, polymer, 190 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
myristoylated neuronal calcium sensor-1 protein: MGKSNSKLKPEVVEELTRKT YFTEKEVQQWYKGFIKDCPS GQLDAAGFQKIYKQFFPFGD PTKFATFVFNVFDENKDGRI EFSEFIQALSVTSRGTLDEK LRWAFKLYDLDNDGYITRNE MLDIVDAIYQMVGNTVELPE EENTPEKRVDRIFAMMDKNA DGKLTLQEFQEGSKADPSIV QALSLYDGLV

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	654
15N chemical shifts	176
1H chemical shifts	1072

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	myristoylated NCS-1	1
2	CALCIUM (II) ION, 1	2
3	CALCIUM (II) ION, 2	2
4	CALCIUM (II) ION, 3	2

Entities:

Entity 1, myristoylated NCS-1 190 residues - Formula weight is not available

1	MET	GLY	LYS	SER	ASN	SER	LYS	LEU	LYS	PRO
2	GLU	VAL	VAL	GLU	GLU	LEU	THR	ARG	LYS	THR
3	TYR	PHE	THR	GLU	LYS	GLU	VAL	GLN	GLN	TRP
4	TYR	LYS	GLY	PHE	ILE	LYS	ASP	CYS	PRO	SER
5	GLY	GLN	LEU	ASP	ALA	ALA	GLY	PHE	GLN	LYS
6	ILE	TYR	LYS	GLN	PHE	PHE	PRO	PHE	GLY	ASP
7	PRO	THR	LYS	PHE	ALA	THR	PHE	VAL	PHE	ASN
8	VAL	PHE	ASP	GLU	ASN	LYS	ASP	GLY	ARG	ILE
9	GLU	PHE	SER	GLU	PHE	ILE	GLN	ALA	LEU	SER
10	VAL	THR	SER	ARG	GLY	THR	LEU	ASP	GLU	LYS
11	LEU	ARG	TRP	ALA	PHE	LYS	LEU	TYR	ASP	LEU
12	ASP	ASN	ASP	GLY	TYR	ILE	THR	ARG	ASN	GLU
13	MET	LEU	ASP	ILE	VAL	ASP	ALA	ILE	TYR	GLN
14	MET	VAL	GLY	ASN	THR	VAL	GLU	LEU	PRO	GLU
15	GLU	GLU	ASN	THR	PRO	GLU	LYS	ARG	VAL	ASP
16	ARG	ILE	PHE	ALA	MET	MET	ASP	LYS	ASN	ALA
17	ASP	GLY	LYS	LEU	THR	LEU	GLN	GLU	PHE	GLN
18	GLU	GLY	SER	LYS	ALA	ASP	PRO	SER	ILE	VAL
19	GLN	ALA	LEU	SER	LEU	TYR	ASP	GLY	LEU	VAL

Entity 2, CALCIUM (II) ION, 1 - Ca - 40.078 Da.

1

CA

Samples:

myr-NCS-1: myristoylated neuronal calcium sensor-1 protein, [U-13C; U-15N], 2 mM; Tris buffer 50 mM; KCl 100 mM; DTT 1 mM

conditions_1: ionic strength: 100 mM; pH: 7.2; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N_HSQC	myr-NCS-1	not available	conditions_1
CBCANH	myr-NCS-1	not available	conditions_1
CBCACONH	myr-NCS-1	not available	conditions_1
1H15N_NOESY-HSQC	myr-NCS-1	not available	conditions_1
HCCH_COSY	myr-NCS-1	not available	conditions_1
HNCO	myr-NCS-1	not available	conditions_1
1H15N_TOCSY-HSQC	myr-NCS-1	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	16334 16726 4378
PDB	1G8I 2LCP 2YOU 2YOV 4GUK 4OV2 4UYC
DBJ	BAC37557 BAG73169
EMBL	CAA57678 CAA58867 CAG05167 CAH90617 CAJ81731
GB	AAA85633 AAA88510 AAC59690 AAD01642 AAF01804
REF	NP_001018350 NP_001035637 NP_001084088 NP_001085136 NP_001122298
SP	P62166 P62167 P62168 Q2V8Y7 Q5RC90
TPG	DAA24173
AlphaFold	P62166 P62167 P62168 Q2V8Y7 Q5RC90

1	MET	GLY	LYS	SER	ASN	SER	LYS	LEU	LYS	PRO
2	GLU	VAL	VAL	GLU	GLU	LEU	THR	ARG	LYS	THR
3	TYR	PHE	THR	GLU	LYS	GLU	VAL	GLN	GLN	TRP
4	TYR	LYS	GLY	PHE	ILE	LYS	ASP	CYS	PRO	SER
5	GLY	GLN	LEU	ASP	ALA	ALA	GLY	PHE	GLN	LYS
6	ILE	TYR	LYS	GLN	PHE	PHE	PRO	PHE	GLY	ASP
7	PRO	THR	LYS	PHE	ALA	THR	PHE	VAL	PHE	ASN
8	VAL	PHE	ASP	GLU	ASN	LYS	ASP	GLY	ARG	ILE
9	GLU	PHE	SER	GLU	PHE	ILE	GLN	ALA	LEU	SER
10	VAL	THR	SER	ARG	GLY	THR	LEU	ASP	GLU	LYS
11	LEU	ARG	TRP	ALA	PHE	LYS	LEU	TYR	ASP	LEU
12	ASP	ASN	ASP	GLY	TYR	ILE	THR	ARG	ASN	GLU
13	MET	LEU	ASP	ILE	VAL	ASP	ALA	ILE	TYR	GLN
14	MET	VAL	GLY	ASN	THR	VAL	GLU	LEU	PRO	GLU
15	GLU	GLU	ASN	THR	PRO	GLU	LYS	ARG	VAL	ASP
16	ARG	ILE	PHE	ALA	MET	MET	ASP	LYS	ASN	ALA
17	ASP	GLY	LYS	LEU	THR	LEU	GLN	GLU	PHE	GLN
18	GLU	GLY	SER	LYS	ALA	ASP	PRO	SER	ILE	VAL
19	GLN	ALA	LEU	SER	LEU	TYR	ASP	GLY	LEU	VAL

1	MET	GLY	LYS	SER	ASN	SER	LYS	LEU	LYS	PRO
2	GLU	VAL	VAL	GLU	GLU	LEU	THR	ARG	LYS	THR
3	TYR	PHE	THR	GLU	LYS	GLU	VAL	GLN	GLN	TRP
4	TYR	LYS	GLY	PHE	ILE	LYS	ASP	CYS	PRO	SER
5	GLY	GLN	LEU	ASP	ALA	ALA	GLY	PHE	GLN	LYS
6	ILE	TYR	LYS	GLN	PHE	PHE	PRO	PHE	GLY	ASP
7	PRO	THR	LYS	PHE	ALA	THR	PHE	VAL	PHE	ASN
8	VAL	PHE	ASP	GLU	ASN	LYS	ASP	GLY	ARG	ILE
9	GLU	PHE	SER	GLU	PHE	ILE	GLN	ALA	LEU	SER
10	VAL	THR	SER	ARG	GLY	THR	LEU	ASP	GLU	LYS
11	LEU	ARG	TRP	ALA	PHE	LYS	LEU	TYR	ASP	LEU
12	ASP	ASN	ASP	GLY	TYR	ILE	THR	ARG	ASN	GLU
13	MET	LEU	ASP	ILE	VAL	ASP	ALA	ILE	TYR	GLN
14	MET	VAL	GLY	ASN	THR	VAL	GLU	LEU	PRO	GLU
15	GLU	GLU	ASN	THR	PRO	GLU	LYS	ARG	VAL	ASP
16	ARG	ILE	PHE	ALA	MET	MET	ASP	LYS	ASN	ALA
17	ASP	GLY	LYS	LEU	THR	LEU	GLN	GLU	PHE	GLN
18	GLU	GLY	SER	LYS	ALA	ASP	PRO	SER	ILE	VAL
19	GLN	ALA	LEU	SER	LEU	TYR	ASP	GLY	LEU	VAL