BMRB Entry 4378

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PDB ID: 2lcp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4378
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N Resonance Assignment of un-myristoylated Ca2+-Frequenin, a Synaptic Efficacy Modulator

Deposition date:

1999-08-18

Original release date:

2000-03-13

Authors:

Kragelund, Birthe; Hauenschild, Alexander; Carlstrom, Goran; Pongs, Olaf; Finn, Bryan

Citation:

Citation: Kragelund, Birthe; Hauenschild, Alexander; Carlstrom, Goran; Pongs, Olaf; Finn, Bryan. "Letter to the Editor: 1H, 13C, and 15N assignments of un-myristoylated Ca2+-frequenin, a synaptic efficacy modulator" J. Biomol. NMR 16, 85-86 (2000).

Assembly members:

Assembly members:
calcium loaded un-myristoylated rat frequenin, polymer, 190 residues, 21747.56 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
calcium loaded un-myristoylated rat frequenin: MGKSNSKLKPEVVEELTRKT YFTEKEVQQWYKGFIKDCPS GQLDAAGFQKIYKQFFPFGD PTKFATFVFNVFDENKDGRI EFSEFIQALSVTSRGTLDEK LRWAFKLYDLDNDGYITRNE MLDIVDAIYQMVGNTVELPE EENTPEKRVDRIFAMMDKNA DGKLTLQEFQEGSKADPSIV QALSLYDGLV

Data sets:

assigned_chemical_shifts
- A

Data type	Count
13C chemical shifts	721
15N chemical shifts	182
1H chemical shifts	1075

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	rfrq	1
2	Ca-1	2
3	Ca-2	2
4	Ca-3	2

Entities:

Entity 1, rfrq 190 residues - 21747.56 Da.

1	MET	GLY	LYS	SER	ASN	SER	LYS	LEU	LYS	PRO
2	GLU	VAL	VAL	GLU	GLU	LEU	THR	ARG	LYS	THR
3	TYR	PHE	THR	GLU	LYS	GLU	VAL	GLN	GLN	TRP
4	TYR	LYS	GLY	PHE	ILE	LYS	ASP	CYS	PRO	SER
5	GLY	GLN	LEU	ASP	ALA	ALA	GLY	PHE	GLN	LYS
6	ILE	TYR	LYS	GLN	PHE	PHE	PRO	PHE	GLY	ASP
7	PRO	THR	LYS	PHE	ALA	THR	PHE	VAL	PHE	ASN
8	VAL	PHE	ASP	GLU	ASN	LYS	ASP	GLY	ARG	ILE
9	GLU	PHE	SER	GLU	PHE	ILE	GLN	ALA	LEU	SER
10	VAL	THR	SER	ARG	GLY	THR	LEU	ASP	GLU	LYS
11	LEU	ARG	TRP	ALA	PHE	LYS	LEU	TYR	ASP	LEU
12	ASP	ASN	ASP	GLY	TYR	ILE	THR	ARG	ASN	GLU
13	MET	LEU	ASP	ILE	VAL	ASP	ALA	ILE	TYR	GLN
14	MET	VAL	GLY	ASN	THR	VAL	GLU	LEU	PRO	GLU
15	GLU	GLU	ASN	THR	PRO	GLU	LYS	ARG	VAL	ASP
16	ARG	ILE	PHE	ALA	MET	MET	ASP	LYS	ASN	ALA
17	ASP	GLY	LYS	LEU	THR	LEU	GLN	GLU	PHE	GLN
18	GLU	GLY	SER	LYS	ALA	ASP	PRO	SER	ILE	VAL
19	GLN	ALA	LEU	SER	LEU	TYR	ASP	GLY	LEU	VAL

Entity 2, Ca-1 - Ca - 40.078 Da.

1

CA

Samples:

sample_one: calcium loaded un-myristoylated rat frequenin, [U-99% 15N], 1.4 mM; CaCl2 8 mM; DTT 14 mM; NaN3 0.2%; D2O 10%; H2O 90%

sample_two: calcium loaded un-myristoylated rat frequenin, [U-99% 13C], 1.4 mM; CaCl2 8 mM; DTT 14 mM; NaN3 0.2%; D2O 10%; H2O 90%

sample_three: calcium loaded un-myristoylated rat frequenin, [U-99% 13C; U-99% 15N], 1.4 mM; CaCl2 8 mM; DTT 14 mM; NaN3 0.2%; D2O 10%; H2O 90%

sample_four: calcium loaded un-myristoylated rat frequenin, [U-99% 13C], 1.4 mM; CaCl2 8 mM; DTT 14 mM; NaN3 0.2%; D2O 100%

sample_conditions_one: pH: 7.2 na; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY-HSQC	not available	not available	sample_conditions_one
3D 1H-15N TOCSY-HSQC	not available	not available	sample_conditions_one
3D HNCA	not available	not available	sample_conditions_one
3D HNCO	not available	not available	sample_conditions_one
3D HNCOCA	not available	not available	sample_conditions_one
3D CBCACOHN	not available	not available	sample_conditions_one
3D CBCACOHA	not available	not available	sample_conditions_one
3D HCCH-TOCSY	not available	not available	sample_conditions_one
3D 1H-13C NOESY-HSQC	not available	not available	sample_conditions_one
4D 1H-13C NOESY-HSQC	not available	not available	sample_conditions_one
2D 1H-15N HSQC	not available	not available	sample_conditions_one

Software:

PRONTO v19981230, Carlsberg A/S - assignment

NMR spectrometers:

Varian UNITYplus 600 MHz

BMRB	16334 16726
PDB	1G8I 2LCP 4GUK 4OV2 4UYC 4YRU 5AEQ 5AER 5AFP
DBJ	BAC37557 BAG73169
EMBL	CAA57678 CAA58867 CAG05167 CAH90617 CAJ81731
GB	AAA85633 AAA88510 AAC59690 AAD01642 AAF01804
REF	NP_001018350 NP_001035637 NP_001084088 NP_001085136 NP_001122298
SP	P62166 P62167 P62168 Q2V8Y7 Q5RC90
TPG	DAA24173
AlphaFold	P62166 P62167 P62168 Q2V8Y7 Q5RC90

1	MET	GLY	LYS	SER	ASN	SER	LYS	LEU	LYS	PRO
2	GLU	VAL	VAL	GLU	GLU	LEU	THR	ARG	LYS	THR
3	TYR	PHE	THR	GLU	LYS	GLU	VAL	GLN	GLN	TRP
4	TYR	LYS	GLY	PHE	ILE	LYS	ASP	CYS	PRO	SER
5	GLY	GLN	LEU	ASP	ALA	ALA	GLY	PHE	GLN	LYS
6	ILE	TYR	LYS	GLN	PHE	PHE	PRO	PHE	GLY	ASP
7	PRO	THR	LYS	PHE	ALA	THR	PHE	VAL	PHE	ASN
8	VAL	PHE	ASP	GLU	ASN	LYS	ASP	GLY	ARG	ILE
9	GLU	PHE	SER	GLU	PHE	ILE	GLN	ALA	LEU	SER
10	VAL	THR	SER	ARG	GLY	THR	LEU	ASP	GLU	LYS
11	LEU	ARG	TRP	ALA	PHE	LYS	LEU	TYR	ASP	LEU
12	ASP	ASN	ASP	GLY	TYR	ILE	THR	ARG	ASN	GLU
13	MET	LEU	ASP	ILE	VAL	ASP	ALA	ILE	TYR	GLN
14	MET	VAL	GLY	ASN	THR	VAL	GLU	LEU	PRO	GLU
15	GLU	GLU	ASN	THR	PRO	GLU	LYS	ARG	VAL	ASP
16	ARG	ILE	PHE	ALA	MET	MET	ASP	LYS	ASN	ALA
17	ASP	GLY	LYS	LEU	THR	LEU	GLN	GLU	PHE	GLN
18	GLU	GLY	SER	LYS	ALA	ASP	PRO	SER	ILE	VAL
19	GLN	ALA	LEU	SER	LEU	TYR	ASP	GLY	LEU	VAL

1	MET	GLY	LYS	SER	ASN	SER	LYS	LEU	LYS	PRO
2	GLU	VAL	VAL	GLU	GLU	LEU	THR	ARG	LYS	THR
3	TYR	PHE	THR	GLU	LYS	GLU	VAL	GLN	GLN	TRP
4	TYR	LYS	GLY	PHE	ILE	LYS	ASP	CYS	PRO	SER
5	GLY	GLN	LEU	ASP	ALA	ALA	GLY	PHE	GLN	LYS
6	ILE	TYR	LYS	GLN	PHE	PHE	PRO	PHE	GLY	ASP
7	PRO	THR	LYS	PHE	ALA	THR	PHE	VAL	PHE	ASN
8	VAL	PHE	ASP	GLU	ASN	LYS	ASP	GLY	ARG	ILE
9	GLU	PHE	SER	GLU	PHE	ILE	GLN	ALA	LEU	SER
10	VAL	THR	SER	ARG	GLY	THR	LEU	ASP	GLU	LYS
11	LEU	ARG	TRP	ALA	PHE	LYS	LEU	TYR	ASP	LEU
12	ASP	ASN	ASP	GLY	TYR	ILE	THR	ARG	ASN	GLU
13	MET	LEU	ASP	ILE	VAL	ASP	ALA	ILE	TYR	GLN
14	MET	VAL	GLY	ASN	THR	VAL	GLU	LEU	PRO	GLU
15	GLU	GLU	ASN	THR	PRO	GLU	LYS	ARG	VAL	ASP
16	ARG	ILE	PHE	ALA	MET	MET	ASP	LYS	ASN	ALA
17	ASP	GLY	LYS	LEU	THR	LEU	GLN	GLU	PHE	GLN
18	GLU	GLY	SER	LYS	ALA	ASP	PRO	SER	ILE	VAL
19	GLN	ALA	LEU	SER	LEU	TYR	ASP	GLY	LEU	VAL