BMRB Entry 6918

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for CRC-N (algal centrin N-term)
Published: 2007-01-29

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6918

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for CRC-N (algal centrin N-term)

Deposition date:

2005-12-06

Original release date:

2007-01-29

Authors:

Sheehan, Jonathan; Bunick, Christopher; Hu, Haitao; Fagan, Patricia; Meyn, Susan; Chazin, Walter

Citation:

Citation: Sheehan, Jonathan; Bunick, Christopher; Hu, Haitao; Fagan, Patricia; Meyn, Susan; Chazin, Walter. "Structure of the N-terminal Calcium Sensor Domain of Centrin Reveals the Biochemical Basis for Domain-Specific Function" J. Biol. Chem. 281, 2876-2881 (2006).
PubMed: 16317001

Assembly members:

Assembly members:
CRC-N, polymer, 96 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: green algae Taxonomy ID: 3055 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Chlamydomonas reinhardtii

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CRC-N: GSMSYKAKTVVSARRDQKKG RVGLTEEQKQEIREAFDLFD TDGSGTIDAKELKVAMRALG FEPKKEEIKKMISEIDKDGS GTIDFEEFLTMMTAKM

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	384
15N chemical shifts	93
1H chemical shifts	659

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	N-terminal domain of caltractin	1
2	CALCIUM (II) ION	2
3	CALCIUM (II) ION	2

Entities:

Entity 1, N-terminal domain of caltractin 96 residues - Formula weight is not available

1

GLY

SER

MET

SER

TYR

LYS

ALA

LYS

THR

VAL

2

VAL

SER

ALA

ARG

ASP

GLN

LYS

GLY

3

ARG

VAL

GLY

LEU

THR

GLU

GLN

LYS

GLN

4

GLU

ILE

ARG

GLU

ALA

PHE

ASP

LEU

PHE

ASP

5

THR

ASP

GLY

SER

GLY

THR

ILE

ASP

ALA

LYS

6

GLU

LEU

LYS

VAL

ALA

MET

ARG

ALA

LEU

GLY

7

PHE

GLU

PRO

LYS

GLU

ILE

LYS

8

MET

ILE

SER

GLU

ILE

ASP

LYS

ASP

GLY

SER

9

GLY

THR

ILE

ASP

PHE

GLU

PHE

LEU

THR

10

MET

THR

ALA

LYS

MET

Entity 2, CALCIUM (II) ION - Ca - 40.078 Da.

1

CA

Samples:

sample_1: CRC-N, [U-13C; U-15N], 1 mM; CaCl25 – 10 mM; Tris-d11 25 mM

conditions_1: pH: 7.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	sample_1	not available	conditions_1
CBCANH	sample_1	not available	conditions_1
CBCA(CO)NH	sample_1	not available	conditions_1
HNCO	sample_1	not available	conditions_1
(H)CC(CO)NH	sample_1	not available	conditions_1
H(CCCO)NH	sample_1	not available	conditions_1
HBHA(CO)NH	sample_1	not available	conditions_1
1H COSY	sample_1	not available	conditions_1
1H 2-quantum	sample_1	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Bruker DRX 600 MHz
Bruker DRX 800 MHz

BMRB	6820
PDB	2AMI