BMRB Entry 6820

Name: Solution Structure Of The Calcium-loaded N-Terminal Sensor Domain Of Centrin
Published: 2006-12-28

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PDB ID: 2ami
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6820
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure Of The Calcium-loaded N-Terminal Sensor Domain Of Centrin

Deposition date:

2005-09-09

Original release date:

2006-12-28

Authors:

Hu, H.; Fagan, P.; Bunick, C.; Sheehan, J.; Chazin, W.

Citation:

Citation: Hu, H.; Fagan, P.; Bunick, C.; Sheehan, J.; Chazin, W.. "Structure of the N-terminal calcium sensor domain of centrin reveals the biochemical basis for domain-specific function." J. Biol. Chem. 281, 2876-2881 (2006).
PubMed: 16317001

Assembly members:

Assembly members:
Caltractin, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Chlamydomonas reinhardtii Taxonomy ID: 3055 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Chlamydomonas reinhardtii

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Caltractin: GSMSYKAKTVVSARRDQKKG RVGLTEEQKQEIREAFDLFD TDGSGTIDAKELKVAMRALG FEPKKEEIKKMISEIDKDGS GTIDFEEFLTMMTAKM

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	382
15N chemical shifts	93
1H chemical shifts	662

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Caltractin	1

Entities:

Entity 1, Caltractin 96 residues - Formula weight is not available

1

GLY

SER

MET

SER

TYR

LYS

ALA

LYS

THR

VAL

2

VAL

SER

ALA

ARG

ASP

GLN

LYS

GLY

3

ARG

VAL

GLY

LEU

THR

GLU

GLN

LYS

GLN

4

GLU

ILE

ARG

GLU

ALA

PHE

ASP

LEU

PHE

ASP

5

THR

ASP

GLY

SER

GLY

THR

ILE

ASP

ALA

LYS

6

GLU

LEU

LYS

VAL

ALA

MET

ARG

ALA

LEU

GLY

7

PHE

GLU

PRO

LYS

GLU

ILE

LYS

8

MET

ILE

SER

GLU

ILE

ASP

LYS

ASP

GLY

SER

9

GLY

THR

ILE

ASP

PHE

GLU

PHE

LEU

THR

10

MET

THR

ALA

LYS

MET

Samples:

sample_1: Caltractin1 – 2 mM; Tris-d11 25 mM; CaCl25 – 10 mM; H2O 90%; D2O 10%

sample_2: Caltractin, [U-15N], 1 – 2 mM; Tris-d11 25 mM; CaCl25 – 10 mM; H2O 90%; D2O 10%

sample_3: Caltractin, [U-13C], 1 – 2 mM; Tris-d11 25 mM; CaCl25 – 10 mM; H2O 90%; D2O 10%

sample_4: Caltractin, [U-13C; U-15N], 1 – 2 mM; Tris-d11 25 mM; CaCl25 – 10 mM; H2O 90%; D2O 10%

sample_5: Caltractin, [U-10% 13C], 1 – 2 mM; Tris-d11 25 mM; CaCl25 – 10 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 7.5 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	not available
3D 15N-NOESY-HSQC	not available	not available	not available
3D 13C-NOESY-HSQC	not available	not available	not available
4D 13C-HMQC-NOESY-HMQC	not available	not available	not available

Software:

FELIX v2000 - processing

SANE v1 - processing

DYANA v1.5 - structure solution

AMBER v7.0 - refinement

xwinnmr v2.6 - collection

NMR spectrometers:

Bruker DRX 800 MHz