BMRB Entry 6719
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6719
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Citation: Kato, Y.; Hino, Y.; Nagata, K.; Tanokura, M.. "Solution structure and binding specificity of FBP11/HYPA WW domain as
Group-II/III" Proteins 63, 227-234 (2006).
PubMed: 16463264
Assembly members:
FBP11 WW1, polymer, 30 residues, Formula weight is not available
PL PEPTIDE, polymer, 9 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
FBP11 WW1: GSWTEHKSPDGRTYYYNTET
KQSTWEKPDD
PL PEPTIDE: GSTAPPLPR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 214 |
| 13C chemical shifts | 104 |
| 15N chemical shifts | 31 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | huntingtin-interacting protein HYPA/FBP11 | 1 |
| 2 | PL (PPLP) motif peptide from Bbc1 prtein | 2 |
Entities:
Entity 1, huntingtin-interacting protein HYPA/FBP11 30 residues - Formula weight is not available
| 1 | GLY | SER | TRP | THR | GLU | HIS | LYS | SER | PRO | ASP | |
| 2 | GLY | ARG | THR | TYR | TYR | TYR | ASN | THR | GLU | THR | |
| 3 | LYS | GLN | SER | THR | TRP | GLU | LYS | PRO | ASP | ASP |
Entity 2, PL (PPLP) motif peptide from Bbc1 prtein 9 residues - Formula weight is not available
| 1 | GLY | SER | THR | ALA | PRO | PRO | LEU | PRO | ARG |
Samples:
sample_1: FBP11 WW1, [U-15N; U-13C], 1.5 mM; PL PEPTIDE 5.3 mM; phosphate buffer 50 mM; NaCl 50 mM; H2O 90%; D2O 10%
sample_2: FBP11 WW1, [U-15N; U-13C], 1.5 mM; PL PEPTIDE 5.3 mM; phosphate buffer 50 mM; NaCl 50 mM; D2O 100%
sample_3: FBP11 WW1 1.6 mM; PL PEPTIDE, [U-15N; U-13C], 4.4 mM; phosphate buffer 50 mM; NaCl 50 mM; H2O 90%; D2O 10%
sample_4: FBP11 WW1 1.6 mM; PL PEPTIDE, [U-15N; U-13C], 4.4 mM; phosphate buffer 50 mM; NaCl 50 mM; D2O 100%
sample_cond_1: pH: 5.0; temperature: 283 K; ionic strength: 0.3 M; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | not available | not available | not available |
| HNHA | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
Software:
CYANA v2.0 - structure solution, refinement
NMRPipe - processing
SPARKY v3 - data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks