BMRB Entry 6719

Title:
Complex structure of the first WW domain of FBP11/HYPA and a -Pro-Pro-Leu-Pro- ligand reveals mechanism of Pro-rich ligand recognition by Group-II/III WW domains
Deposition date:
2005-06-21
Original release date:
2007-01-29
Authors:
Kato, Y.; Miyakawa, T.; Kurita, J.; Tanokura, M.
Citation:

Citation: Kato, Y.; Hino, Y.; Nagata, K.; Tanokura, M.. "Solution structure and binding specificity of FBP11/HYPA WW domain as Group-II/III"  Proteins 63, 227-234 (2006).
PubMed: 16463264

Assembly members:

Assembly members:
FBP11 WW1, polymer, 30 residues, Formula weight is not available
PL PEPTIDE, polymer, 9 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FBP11 WW1: GSWTEHKSPDGRTYYYNTET KQSTWEKPDD
PL PEPTIDE: GSTAPPLPR

Data sets:
Data typeCount
1H chemical shifts214
13C chemical shifts104
15N chemical shifts31

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1huntingtin-interacting protein HYPA/FBP111
2PL (PPLP) motif peptide from Bbc1 prtein2

Entities:

Entity 1, huntingtin-interacting protein HYPA/FBP11 30 residues - Formula weight is not available

1   GLYSERTRPTHRGLUHISLYSSERPROASP
2   GLYARGTHRTYRTYRTYRASNTHRGLUTHR
3   LYSGLNSERTHRTRPGLULYSPROASPASP

Entity 2, PL (PPLP) motif peptide from Bbc1 prtein 9 residues - Formula weight is not available

1   GLYSERTHRALAPROPROLEUPROARG

Samples:

sample_1: FBP11 WW1, [U-15N; U-13C], 1.5 mM; PL PEPTIDE 5.3 mM; phosphate buffer 50 mM; NaCl 50 mM; H2O 90%; D2O 10%

sample_2: FBP11 WW1, [U-15N; U-13C], 1.5 mM; PL PEPTIDE 5.3 mM; phosphate buffer 50 mM; NaCl 50 mM; D2O 100%

sample_3: FBP11 WW1 1.6 mM; PL PEPTIDE, [U-15N; U-13C], 4.4 mM; phosphate buffer 50 mM; NaCl 50 mM; H2O 90%; D2O 10%

sample_4: FBP11 WW1 1.6 mM; PL PEPTIDE, [U-15N; U-13C], 4.4 mM; phosphate buffer 50 mM; NaCl 50 mM; D2O 100%

sample_cond_1: pH: 5.0; temperature: 283 K; ionic strength: 0.3 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available

Software:

CYANA v2.0 - structure solution, refinement

NMRPipe - processing

SPARKY v3 - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GenBank AAC27502
BMRB 6721 6558 6559

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks