BMRB Entry 6721

Name: Solution structure of the first WW domain of FBP11
Published: 2006-04-24

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PDB ID: 1zr7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6721
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the first WW domain of FBP11

Deposition date:

2005-06-30

Original release date:

2006-04-24

Authors:

Kato, Y.; Hino, Y.; Tanokura, M.

Citation:

Citation: Kato, Y.; Hino, Y.; Nagata, K.; Tanokura, M.. "Solution structure and binding specificity of FBP11/HYPA WW domain as Group-II/III" Proteins 63, 227-234 (2006).
PubMed: 16463264

Assembly members:

Assembly members:
FBP11 WW1, polymer, 30 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FBP11 WW1: GSWTEHKSPDGRTYYYNTET KQSTWEKPDD

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	188
13C chemical shifts	97
15N chemical shifts	30

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	huntingtin-interacting protein HYPA/FBP11	1

Entities:

Entity 1, huntingtin-interacting protein HYPA/FBP11 30 residues - Formula weight is not available

1	GLY	SER	TRP	THR	GLU	HIS	LYS	SER	PRO	ASP
2	GLY	ARG	THR	TYR	TYR	TYR	ASN	THR	GLU	THR
3	LYS	GLN	SER	THR	TRP	GLU	LYS	PRO	ASP	ASP

Samples:

sample_1: FBP11 WW1, [U-15N; U-13C], 1.5 mM; phosphate buffer 50 mM; NaCl 50 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 5.0; temperature: 298 K; ionic strength: 0.175 M; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	not available	not available	not available
HNHA	not available	not available	not available

Software:

CYANA v2.0 - structure solution, refinement

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	6558
PDB	1YWI 1YWJ 1ZR7 2DYF
GB	AAC27502 EHH21390
REF	XP_007951755

1	GLY	SER	TRP	THR	GLU	HIS	LYS	SER	PRO	ASP
2	GLY	ARG	THR	TYR	TYR	TYR	ASN	THR	GLU	THR
3	LYS	GLN	SER	THR	TRP	GLU	LYS	PRO	ASP	ASP

1	GLY	SER	TRP	THR	GLU	HIS	LYS	SER	PRO	ASP
2	GLY	ARG	THR	TYR	TYR	TYR	ASN	THR	GLU	THR
3	LYS	GLN	SER	THR	TRP	GLU	LYS	PRO	ASP	ASP