BMRB Entry 6718

Name: NMR structure of the human dematin headpiece S74E mutant
Published: 2006-04-24

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PDB ID: 1zv6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6718
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of the human dematin headpiece S74E mutant

Deposition date:

2005-06-30

Original release date:

2006-04-24

Authors:

Jiang, Z.; Mcknight, C.

Citation:

Citation: Jiang, Z.; Mcknight, C.. "A phosphorylation-induced conformation change in dematin headpiece" Structure 14, 379-87 (2005).
PubMed: 16472756

Assembly members:

Assembly members:
DHPs74e, polymer, 68 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DHPs74e: PGLQIYPYEMLVVTNKGRTK LPPGVDRMRLERHLSAEDFS RVFAMSPEEFGKLALWKRNE LKKKAELF

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	290
15N chemical shifts	70
1H chemical shifts	521

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DHPs74e protein	1

Entities:

Entity 1, DHPs74e protein 68 residues - Formula weight is not available

1

PRO

GLY

LEU

GLN

ILE

TYR

PRO

TYR

GLU

MET

2

LEU

VAL

THR

ASN

LYS

GLY

ARG

THR

LYS

3

LEU

PRO

GLY

VAL

ASP

ARG

MET

ARG

LEU

4

GLU

ARG

HIS

LEU

SER

ALA

GLU

ASP

PHE

SER

5

ARG

VAL

PHE

ALA

MET

SER

PRO

GLU

PHE

6

GLY

LYS

LEU

ALA

LEU

TRP

LYS

ARG

ASN

GLU

7

LEU

LYS

ALA

GLU

LEU

PHE

Samples:

sample_1: DHPs74e, [U-13C; U-15N], 1 mM; PHOSPHATE 10 mM; H2O 90%; D2O 10%

sample_2: DHPs74e, [U-15N], 1 mM; PHOSPHATE 10 mM; H2O 90%; D2O 10%

sample_3: DHPs74e, [U-10% 13C; U-15N], 1 mM; PHOSPHATE 10 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 10 mM; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	not available	not available	not available
HNCA-J	not available	not available	not available
HNCOCA	not available	not available	not available
2D NOESY	not available	not available	not available
3D 15N-separated NOESY	not available	not available	not available
HNHA	not available	not available	not available
HNHB	not available	not available	not available
2D TOCSY	not available	not available	not available

Software:

Molmol v2K.2 - structure solution

CNS v1.0 - refinement

NMRPipe v2004 version - processing

xwinnmr v3.1 - collection

NMRView v5.0.4. - data analysis

NMR spectrometers:

BRUKER DMX 500 MHz

BMRB	5968
PDB	1QZP 1ZV6
DBJ	BAD92955 BAE28799 BAE34638 BAE37092 BAE38123
EMBL	CAH92510 CAH93265 CAH93400
GB	AAC50223 AAD34233 AAD38412 AAH06318 AAH16897
REF	NP_001107607 NP_001107608 NP_001107609 NP_001107610 NP_001107611
SP	Q08495 Q5R4B6 Q9WV69
AlphaFold	Q08495 Q5R4B6 Q9WV69