BMRB Entry 6619
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6619
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Citation: Martins, Rafael; Sforca, Mauricio; Amino, Rogerio; Juliano, Maria; Oyama, Sergio; Juliano, Luiz; Pertinhez, Thelma; Spisni, Alberto; Schenkman, Sergio. "Lytic Activity and Structural Differences of Amphipathic Peptides Derived from
Trialysin" Biochemistry 45, 1765-1774 (2006).
PubMed: 16460023
Assembly members:
P7, polymer, 27 residues, 3041.8 Da.
Natural source: Common Name: Triatoma infestans Taxonomy ID: 30076 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Triatoma infestans
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
P7: FKIKPGKVLDKFGKIVGKVL
KQLKKVS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 153 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | P7 | 1 |
Entities:
Entity 1, P7 27 residues - 3041.8 Da.
| 1 | PHE | LYS | ILE | LYS | PRO | GLY | LYS | VAL | LEU | ASP | ||||
| 2 | LYS | PHE | GLY | LYS | ILE | VAL | GLY | LYS | VAL | LEU | ||||
| 3 | LYS | GLN | LEU | LYS | LYS | VAL | SER |
Samples:
sample_1: P7 1.2 mM; TFE 30 % (vol); D2O 5 % (vol); H2O 65 % (vol)
P7_conditions: pH: 4; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H TOCSY | sample_1 | not available | P7_conditions |
| 1H COSY | sample_1 | not available | P7_conditions |
| 1H NOESY | sample_1 | not available | P7_conditions |
| 1H ROESY | sample_1 | not available | P7_conditions |
Software:
NMRView5 v5, One Moon Scientific Inc. - chemical shift assignment, spectrum analysis
NMR spectrometers:
- Varian Unity 500 MHz