Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6533
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Citation: Ono, Shusuke; Leonard, Paul; Williams, Mark; Ladbury, John. "Characterization of the bacterial nucleoid protein STPA: Structure, self-association and interaction with H-NS" The BMRB entry is the only known published source for the data..
Assembly members:
DNA binding domain of STPA, polymer, 44 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: Not applicable Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Vector: pET14b
Entity Sequences (FASTA):
DNA binding domain of STPA: QPRPAKYKFTDVNGETKTWT
GQGRTPKPIAQALAEGKSLD
DFLI
Data type | Count |
13C chemical shifts | 198 |
15N chemical shifts | 44 |
1H chemical shifts | 313 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | DNA binidng domain of STPA | 1 |
Entity 1, DNA binidng domain of STPA 44 residues - Formula weight is not available
Residues 91 to 134 of the DNA packaing protein STPA
1 | GLN | PRO | ARG | PRO | ALA | LYS | TYR | LYS | PHE | THR | ||||
2 | ASP | VAL | ASN | GLY | GLU | THR | LYS | THR | TRP | THR | ||||
3 | GLY | GLN | GLY | ARG | THR | PRO | LYS | PRO | ILE | ALA | ||||
4 | GLN | ALA | LEU | ALA | GLU | GLY | LYS | SER | LEU | ASP | ||||
5 | ASP | PHE | LEU | ILE |
sample_1: DNA binding domain of STPA, [U-98% 13C; U-98% 15N], 1.5 ± 0.1 mM; sodium chloride 100 ± 5 mM; sodium phosphate 10 ± 0.5 mM; EDTA 0.1 ± 0.05 mM
conditions_1: pH*: 6.5; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1H15N_HSQC | sample_1 | not available | conditions_1 |
1H13C_CT_HSQC | sample_1 | not available | conditions_1 |
HNCACB | sample_1 | not available | conditions_1 |
C(CO)HN | sample_1 | not available | conditions_1 |
CBCA(CO)NH | sample_1 | not available | conditions_1 |
HNCO | sample_1 | not available | conditions_1 |
HCCH-TOCSY | sample_1 | not available | conditions_1 |
HC(CO)NH | sample_1 | not available | conditions_1 |
HBCD | sample_1 | not available | conditions_1 |
1H15N_TOCSY_HSQC | sample_1 | not available | conditions_1 |
1H15N_NOESY_HSQC | sample_1 | not available | conditions_1 |
NMRPipe v2.1, Frank Delaglio, NIH - spectral processing
ANSIG v3.3, University of Cambridge - spectral visualisation
BMRB | 18400 |
PDB | |
DBJ | BAA16535 BAB36953 BAG78446 BAI26931 BAI31961 |
EMBL | CAA49146 CAP77108 CAQ33006 CAQ87964 CAQ99591 |
GB | AAA64940 AAC75716 AAG57777 AAN44190 AAN81670 |
REF | NP_289219 NP_311557 NP_417155 NP_708483 NP_755100 |
SP | P0ACG1 P0ACG2 P0ACG3 |
AlphaFold | P0ACG1 P0ACG2 P0ACG3 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks