BMRB Entry 18400

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18400
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
RNA structural dynamics are modulated through anti-folding by chaperones
Deposition date:
2012-04-15
Original release date:
2012-05-31
Authors:
Fuertig, Boris; Doetsch, Martina; Stampfl, Sabine; Kontaxis, Georg; Schroeder, Renee
Citation:

Citation: Fuertig, Boris; Doetsch, Martina; Stampfl, Sabine; Kontaxis, Georg; Schroeder, Renee. "Chaperones modulate RNA structural dynamics through anti-folding"  .

Assembly members:

Assembly members:
CTD-StpA, polymer, 45 residues, 5039.755 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTWIN-StpA_CTD

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CTD-StpA: RQPRPAKYKFTDVNGETKTW TGQGRTPKPIAQALAEGKSL DDFLI

Data sets:
Data typeCount
13C chemical shifts182
15N chemical shifts43
1H chemical shifts309

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CTD-StpA1

Entities:

Entity 1, CTD-StpA 45 residues - 5039.755 Da.

1   ARGGLNPROARGPROALALYSTYRLYSPHE
2   THRASPVALASNGLYGLUTHRLYSTHRTRP
3   THRGLYGLNGLYARGTHRPROLYSPROILE
4   ALAGLNALALEUALAGLUGLYLYSSERLEU
5   ASPASPPHELEUILE

Samples:

sample_1: CTD-StpA, [U-99% 13C; U-99% 15N], 1 mM

sample_conditions_1: ionic strength: 0.075 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ BAA16535 BAB36953 BAG78446 BAI26931 BAI31961
EMBL CAA49146 CAP77108 CAQ33006 CAQ87964 CAQ99591
GB AAA64940 AAC75716 AAG57777 AAN44190 AAN81670
REF NP_311557 NP_417155 NP_708483 WP_000055597 WP_000115370
SP P0ACG1 P0ACG2 P0ACG3
AlphaFold P0ACG1 P0ACG2 P0ACG3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks