BMRB Entry 6525

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6525

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Title:
Backbone 1H Chemical Shift Assignments for Mungbean Defensin, VrD1
Deposition date:
2005-02-28
Original release date:
2006-04-24
Authors:
Liu, Yaw-Jen; Lyu, Ping-Chiang
Citation:

Citation: Liu, Yaw-Jen; Cheng, Chao-Sheng; Lai, Szu-Ming; Hsu, Ming-Pin; Chen, Ching-San; Lyu, Ping-Chiang. "Solution structure of the plant defensin VrD1 from mung bean and its possible role in insecticidal activity against bruchids"  Proteins 63, 777-786 (2006).
PubMed: 16544327

Assembly members:

Assembly members:
Mungbean defensin, VrD1, polymer, 46 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mung bean   Taxonomy ID: 157791   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Vigna radiata

Experimental source:

Experimental source:   Production method: Purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Mungbean defensin, VrD1: RTCMIKKEGWGKCLIDTTCA HSCKNRGYIGGNCKGMTRTC YCLVNC

Data sets:
Data typeCount
1H chemical shifts281

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Mungbean Defensin, VrD11

Entities:

Entity 1, Mungbean Defensin, VrD1 46 residues - Formula weight is not available

1   ARGTHRCYSMETILELYSLYSGLUGLYTRP
2   GLYLYSCYSLEUILEASPTHRTHRCYSALA
3   HISSERCYSLYSASNARGGLYTYRILEGLY
4   GLYASNCYSLYSGLYMETTHRARGTHRCYS
5   TYRCYSLEUVALASNCYS

Samples:

sample_1: Mungbean Defensin, VrD1 2.0 mM

conditions_1: pH: 6.0; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYsample_1not availableconditions_1
TOCSYsample_1not availableconditions_1
NOESYsample_1not availableconditions_1

Software:

Xwin-nmr v2.1 -

NMR spectrometers:

  • Bruker Avance 600 MHz