BMRB Entry 6441

Name: Chemical shift assignments for human Orexin-A
Published: 2006-04-17

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6441

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shift assignments for human Orexin-A

Deposition date:

2004-12-22

Original release date:

2006-04-17

Authors:

Ikegami, Takahisa; Takai, Tomoyo

Citation:

Citation: Takai, Tomoyo; Takaya, Takao; Nakano, Mutsuko; Akutsu, Hideo; Nakagawa, Atsushi; Aimoto, Saburo; Nagai, Katsuya; Ikegami, Takahisa. "Orexin-A is composed of a highly conserved C-terminal and a specific, hydrophilic N-terminal region, revealing the structural basis of specific recognition by the orexin-1 receptor." J. Pept. Sci. 12, 443-454 (2006).
PubMed: 16429482

Assembly members:

Assembly members:
human Orexin-A, polymer, 33 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human Orexin-A: XPLPDCCRQKTCSCRLYELL HGAGNHAAGILTX

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	54
15N chemical shifts	31
1H chemical shifts	181

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	human Orexin-A	1

Entities:

Entity 1, human Orexin-A 33 residues - Formula weight is not available

1

PCA

PRO

LEU

PRO

ASP

CYS

ARG

GLN

LYS

2

THR

CYS

SER

CYS

ARG

LEU

TYR

GLU

LEU

3

HIS

GLY

ALA

GLY

ASN

HIS

ALA

GLY

ILE

4

LEU

THR

NLW

Samples:

sample_1: human Orexin-A 0.76 mM

conditions_1: ionic strength: 18 mM; pH: 6.0; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D TOCSY	sample_1	not available	conditions_1
2D NOESY	sample_1	not available	conditions_1
DQF-COSY	sample_1	not available	conditions_1
15N-1H-HSQC	sample_1	not available	conditions_1
13C-1H-HSQC	sample_1	not available	conditions_1

Software:

Sparky - chemical shift assignment

NMR spectrometers:

Bruker DRX 800 MHz
Bruker DRX 600 MHz
Bruker DRX 500 MHz

BMRB	5994
PDB	1R02 1WSO
DBJ	BAB91446.1
EMBL	CAM19469.1 CAM19468.1
GenBank	AAL17613.1 AAC26827.1 ABJ15704.1 ABO15568.1 EDM06064.1
REF	XP_001166578.1 NP_001515.1 NP_999321.1 XP_001108991.1 XP_609741.2
SWISS-PROT	O55232 O55241 Q9GLF6 O43612 O77668