BMRB Entry 5994
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5994
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Kim, H.-Y.; Hong, E.; Kim, J.-I.; Lee, W.. "Solution structure of Human Orexin-A: Regulator of Appetite and Wakefulness" J. Biochem. Mol. Biol. 37, 565-573 (2004).
PubMed: 15479620
Assembly members:
human orexin-A, polymer, 33 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
human orexin-A: QPLPDCCRQKTCSCRLYELL
HGAGNHAAGILTL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 166 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Orexin-A | 1 |
Entities:
Entity 1, Orexin-A 33 residues - Formula weight is not available
| 1 | GLN | PRO | LEU | PRO | ASP | CYS | CYS | ARG | GLN | LYS | ||||
| 2 | THR | CYS | SER | CYS | ARG | LEU | TYR | GLU | LEU | LEU | ||||
| 3 | HIS | GLY | ALA | GLY | ASN | HIS | ALA | ALA | GLY | ILE | ||||
| 4 | LEU | THR | LEU |
Samples:
sample_1: human orexin-A 2 mM; phosphate buffer 50 mM; H2O 90%; D2O 10%
sample_2: human orexin-A 2 mM; phosphate buffer 50 mM; D2O 100%
sample_cond_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
| DQF-COSY | not available | not available | not available |
Software:
CNS v1.0 - refinement, structure solution
XWINNMR v2.0 - processing
NMRPipe v1.7 - processing
Sparky v3.60 - data analysis
NMR spectrometers:
- Bruker DRX 600 MHz