BMRB Entry 6408

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for Two C-terminal C2H2 Zinc Fingers (F5-6) of MTF-1
Deposition date:
2004-12-03
Original release date:
2005-11-14
Authors:
Laity, John; Knudsen, Nathan
Citation:

Citation: Potter, Belinda; Feng, Linda; Parasuram, Priya; Matskevich, Viktor; Wilson, Jed; Andrews, Glen; Laity, John. "The six zinc fingers of metal-responsive element binding transcription factor-1 form stable and quasi-ordered structures with relatively small differences in zinc affinities."  J. Biol. Chem. 280, 28529-28540 (2005).
PubMed: 16055450

Assembly members:

Assembly members:
metal-responsive element-binding transcription factor-1, polymer, 61 residues, 6317.1 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
metal-responsive element-binding transcription factor-1: PFRCDHDGCGKAFAASHHLK THVRTHTGERPFFCPSNGCE KTFSTQYSLKSHMKGHDNKG T

Data sets:
Data typeCount
1H chemical shifts49
13C chemical shifts160
15N chemical shifts49

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MTF-1 subunit 11
2Zn (II) A2
3Zn (II) B2

Entities:

Entity 1, MTF-1 subunit 1 61 residues - 6317.1 Da.

1   PROPHEARGCYSASPHISASPGLYCYSGLY
2   LYSALAPHEALAALASERHISHISLEULYS
3   THRHISVALARGTHRHISTHRGLYGLUARG
4   PROPHEPHECYSPROSERASNGLYCYSGLU
5   LYSTHRPHESERTHRGLNTYRSERLEULYS
6   SERHISMETLYSGLYHISASPASNLYSGLY
7   THR

Entity 2, Zn (II) A - Zn - 65.409 Da.

1   ZN

Samples:

all_samples: metal-responsive element-binding transcription factor-1, [U-98% 13C; U-98% 15N], 0.3 – 0.35 mM; B-mercaptoethanol 5 mM; MES 20 mM; H2O 95%; D2O 5%

cond_1: pH: 6.9; temperature: 293 K; ionic strength: 0.01 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCall_samplesnot availablecond_1
HNCACBall_samplesnot availablecond_1
CBCA(CO)NHall_samplesnot availablecond_1
(HCA)CO(CA)NHall_samplesnot availablecond_1
HNCOall_samplesnot availablecond_1

Software:

NMRView v5.0.4 - Data analysis

NMRPipe v97.027.12.56 - Raw spectral data processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

SWISS-PROT Q07243 Q14872
REF XP_001503611.1 XP_513329.2 NP_032662.2 NP_001030252.2 NP_001102147.1
GenBank EDL80405.1 EDL30349.1 AAH17679.1 AAP31813.1 AAP31812.1
EMBL CAA55363.1 CAH70087.1 CAA50470.1 CAB71344.1
DBJ BAE00336.1 BAC26879.1 BAA22262.1
BMRB 6445 6275 6276

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks