BMRB Entry 6408

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6408

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Two C-terminal C2H2 Zinc Fingers (F5-6) of MTF-1

Deposition date:

2004-12-03

Original release date:

2005-11-14

Authors:

Laity, John; Knudsen, Nathan

Citation:

Citation: Potter, Belinda; Feng, Linda; Parasuram, Priya; Matskevich, Viktor; Wilson, Jed; Andrews, Glen; Laity, John. "The six zinc fingers of metal-responsive element binding transcription factor-1 form stable and quasi-ordered structures with relatively small differences in zinc affinities." J. Biol. Chem. 280, 28529-28540 (2005).
PubMed: 16055450

Assembly members:

Assembly members:
metal-responsive element-binding transcription factor-1, polymer, 61 residues, 6317.1 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
metal-responsive element-binding transcription factor-1: PFRCDHDGCGKAFAASHHLK THVRTHTGERPFFCPSNGCE KTFSTQYSLKSHMKGHDNKG T

Data sets:

assigned_chemical_shifts
- MTF-1_F5-6

Data type	Count
1H chemical shifts	49
13C chemical shifts	160
15N chemical shifts	49

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MTF-1 subunit 1	1
2	Zn (II) A	2
3	Zn (II) B	2

Entities:

Entity 1, MTF-1 subunit 1 61 residues - 6317.1 Da.

1

PRO

PHE

ARG

CYS

ASP

HIS

ASP

GLY

CYS

GLY

2

LYS

ALA

PHE

ALA

SER

HIS

LEU

LYS

3

THR

HIS

VAL

ARG

THR

HIS

THR

GLY

GLU

ARG

4

PRO

PHE

CYS

PRO

SER

ASN

GLY

CYS

GLU

5

LYS

THR

PHE

SER

THR

GLN

TYR

SER

LEU

LYS

6

SER

HIS

MET

LYS

GLY

HIS

ASP

ASN

LYS

GLY

7

THR

Entity 2, Zn (II) A - Zn - 65.409 Da.

1

ZN

Samples:

all_samples: metal-responsive element-binding transcription factor-1, [U-98% 13C; U-98% 15N], 0.3 – 0.35 mM; B-mercaptoethanol 5 mM; MES 20 mM; H2O 95%; D2O 5%

cond_1: pH: 6.9; temperature: 293 K; ionic strength: 0.01 M

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	all_samples	not available	cond_1
HNCACB	all_samples	not available	cond_1
CBCA(CO)NH	all_samples	not available	cond_1
(HCA)CO(CA)NH	all_samples	not available	cond_1
HNCO	all_samples	not available	cond_1

Software:

NMRView v5.0.4 - Data analysis

NMRPipe v97.027.12.56 - Raw spectral data processing

NMR spectrometers:

Varian INOVA 600 MHz

SWISS-PROT	Q07243 Q14872
REF	XP_001503611.1 XP_513329.2 NP_032662.2 NP_001030252.2 NP_001102147.1
GenBank	EDL80405.1 EDL30349.1 AAH17679.1 AAP31813.1 AAP31812.1
EMBL	CAA55363.1 CAH70087.1 CAA50470.1 CAB71344.1
DBJ	BAE00336.1 BAC26879.1 BAA22262.1
BMRB	6445 6275 6276