BMRB Entry 6306

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6306

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Title:
Mainchain proton and nitrogen chemical shifts for the C-domain of human RanGAP1 linked via an isopeptide at Lys524 to Gly97 of human Sumo-1
Deposition date:
2004-08-27
Original release date:
2004-11-29
Authors:
Macauley, Matthew; Errington, Wesley; Okon, Mark; Scharpf, Manuela; Mackereth, Cameron; Schulman, Brenda; McIntosh, Lawrence
Citation:

Citation: Macauley, Matthew; Errington, Wesley; Okon, Mark; Scharpf, Manuela; Mackereth, Cameron; Schulman, Brenda; McIntosh, Lawrence. "Structural and Dynamic Independence of Isopeptide-linked RanGAP1 and SUMO-1"  J. Biol. Chem. 279, 49131-49137 (2004).
PubMed: 15355965

Assembly members:

Assembly members:
Sumoylated RanGAP1, polymer, 172 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Animalia   Genus/species: Homo sapian

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sumoylated RanGAP1: GSHMGEPAPVLSSPPPADVS TFLAFPSPEKLLRLGPKSSV LIAQQTDTSDPEKVVSAFLK VSSVFKDEATVRMAVQDAVD ALMQKAFNSSSFNSNTFLTR LLVHMGLLKSEDKVKAIANL YGPLMALNHMVQQDYFPKAL APLLLAFVTKPNSALESCSF ARHSLLQTLYKV

Data sets:
Data typeCount
1H chemical shifts156
15N chemical shifts154

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Sumoylated RanGAP11

Entities:

Entity 1, Sumoylated RanGAP1 172 residues - Formula weight is not available

1   GLYSERHISMETGLYGLUPROALAPROVAL
2   LEUSERSERPROPROPROALAASPVALSER
3   THRPHELEUALAPHEPROSERPROGLULYS
4   LEULEUARGLEUGLYPROLYSSERSERVAL
5   LEUILEALAGLNGLNTHRASPTHRSERASP
6   PROGLULYSVALVALSERALAPHELEULYS
7   VALSERSERVALPHELYSASPGLUALATHR
8   VALARGMETALAVALGLNASPALAVALASP
9   ALALEUMETGLNLYSALAPHEASNSERSER
10   SERPHEASNSERASNTHRPHELEUTHRARG
11   LEULEUVALHISMETGLYLEULEULYSSER
12   GLUASPLYSVALLYSALAILEALAASNLEU
13   TYRGLYPROLEUMETALALEUASNHISMET
14   VALGLNGLNASPTYRPHEPROLYSALALEU
15   ALAPROLEULEULEUALAPHEVALTHRLYS
16   PROASNSERALALEUGLUSERCYSSERPHE
17   ALAARGHISSERLEULEUGLNTHRLEUTYR
18   LYSVAL

Samples:

sample: Sumoylated RanGAP1; KCl 100 mM; KPhos 10 mM; DTT 2 mM; D2O 10%

sample_conditions: pH: 6.0; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions

Software:

NMRpipe -

NMRView -

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

BMRB 6305
PDB
DBJ BAB47464 BAG10161 BAG63578 BAG64552
EMBL CAA57714 CAG30443 CAK54588 CAK54887
GB AAH04990 AAH14044 AAH41396 AIC55014 EAW60420
REF NP_001265580 NP_002874 XP_004063574 XP_004063575 XP_004063576
SP P46060
AlphaFold P46060

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks