BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6305

Title: Mainchain proton, carbon and nitrogen chemical shifts for the C-domain of human RanGAP1   PubMed: 15355965

Deposition date: 2004-08-27 Original release date: 2004-11-29

Authors: Macauley, Matthew; Errington, Wesley; Okon, Mark; Scharpf, Manuela; Mackereth, Cameron; Schulman, Brenda; McIntosh, Lawrence

Citation: Macauley, Matthew; Errington, Wesley; Okon, Mark; Scharpf, Manuela; Mackereth, Cameron; Schulman, Brenda; McIntosh, Lawrence. "Structural and Dynamic Independence of Isopeptide-linked RanGAP1 and SUMO-1"  J. Biol. Chem. 279, 49131-49137 (2004).

Assembly members:
RanGAP1, polymer, 172 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Animalia   Genus/species: Homo sapian

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):
RanGAP1: GSHMGEPAPVLSSPPPADVS TFLAFPSPEKLLRLGPKSSV LIAQQTDTSDPEKVVSAFLK VSSVFKDEATVTMAVQDAVD ALMQKAFNSSSFNSNTFLTR LLVHMGLLKSEDKVKAIANL YGPLMALNHMVQQDYFPKAL APLLLAFVTKPNSALESCSF ARHSLLQTLYKV

Data sets:
Data typeCount
13C chemical shifts459
15N chemical shifts152
1H chemical shifts152

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RanGAP1 C-domain1

Entities:

Entity 1, RanGAP1 C-domain 172 residues - Formula weight is not available

1   GLYSERHISMETGLYGLUPROALAPROVAL
2   LEUSERSERPROPROPROALAASPVALSER
3   THRPHELEUALAPHEPROSERPROGLULYS
4   LEULEUARGLEUGLYPROLYSSERSERVAL
5   LEUILEALAGLNGLNTHRASPTHRSERASP
6   PROGLULYSVALVALSERALAPHELEULYS
7   VALSERSERVALPHELYSASPGLUALATHR
8   VALTHRMETALAVALGLNASPALAVALASP
9   ALALEUMETGLNLYSALAPHEASNSERSER
10   SERPHEASNSERASNTHRPHELEUTHRARG
11   LEULEUVALHISMETGLYLEULEULYSSER
12   GLUASPLYSVALLYSALAILEALAASNLEU
13   TYRGLYPROLEUMETALALEUASNHISMET
14   VALGLNGLNASPTYRPHEPROLYSALALEU
15   ALAPROLEULEULEUALAPHEVALTHRLYS
16   PROASNSERALALEUGLUSERCYSSERPHE
17   ALAARGHISSERLEULEUGLNTHRLEUTYR
18   LYSVAL

Samples:

sample: RanGAP1; KCl 100 mM; KPhos 10 mM; DTT 2 mM; D2O 10%

sample_conditions: pH: 6.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
15N-editted NOESY-HSQCnot availablenot availablenot available
15N-editted TOCSY-HSQCnot availablenot availablenot available

Software:

NMRpipe -

NMRView -

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

BMRB 6306
PDB
DBJ BAB47464 BAG10161 BAG63578 BAG64552
EMBL CAA57714 CAG30443 CAK54588 CAK54887
GB AAH04990 AAH14044 AAH41396 AIC55014 EAW60420
REF NP_001265580 NP_002874 XP_005261752 XP_005261753 XP_006724352
SP P46060

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts