BMRB Entry 6281
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6281
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Citation: Chen, Xiaocheng; Tomchick, Diana; Kovrigin, Evguenii; Arac, Demet; Machius, Mischa; Sudhof, Thomas; Rizo, Josep. "Three-Dimensional Structure of the Complexin/SNARE Complex" Neuron 33, 397-409 (2002).
PubMed: 11832227
Assembly members:
Synaptobrevin2 SNARE motif, polymer, 65 residues, 7761 Da.
Syntaxin 1A SNARE motif, polymer, 63 residues, 7541 Da.
SNAP-25B N-terminal SNARE motif, polymer, 75 residues, 8688 Da.
SNAP-25B C-terminal SNARE motif, polymer, 66 residues, 7603 Da.
Complexin I (26-83), polymer, 56 residues, 7080 Da.
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Synaptobrevin2 SNARE motif: NRRLQQTQAQVDEVVDIMRV
NVDKVLERDQKLSELDDRAD
ALQAGASQFETSAAKLKRKY
WWKNL
Syntaxin 1A SNARE motif: ALSEIETRHSEIIKLENSIR
ELHDMFMDMAMLVESQGEMI
DRIEYNVEHAVDYVERAVSD
TKK
SNAP-25B N-terminal SNARE motif: GSLEEMQRRADQLADESLES
TRRMLQLVEESKDAGIRTLV
MLDEQGEQLDRVEEGMNHIN
QDMKEAEKNLKDLGW
SNAP-25B C-terminal SNARE motif: GSARENEMDENLEQVSGIIG
NLRHMALDMGNEIDTQNRQI
DRIMEKADSNKTRIDEANQR
ATKMLW
Complexin I (26-83): PDAAKKEEERQEALRQAEEE
RKAKYAKMEAEREVMRQGIR
DKYGIKKKEEREAEAQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 151 |
| 13C chemical shifts | 153 |
| 15N chemical shifts | 151 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Synaptobrevin2 (29-93) | 1 |
| 2 | Syntaxin 1A(191-253) | 2 |
| 3 | SNAP-25B (11-82-W) | 3 |
| 4 | SNAP-25B (141-203-W) | 4 |
| 5 | Complexin I(26-83) | 5 |
Entities:
Entity 1, Synaptobrevin2 (29-93) 65 residues - 7761 Da.
| 1 | ASN | ARG | ARG | LEU | GLN | GLN | THR | GLN | ALA | GLN | ||||
| 2 | VAL | ASP | GLU | VAL | VAL | ASP | ILE | MET | ARG | VAL | ||||
| 3 | ASN | VAL | ASP | LYS | VAL | LEU | GLU | ARG | ASP | GLN | ||||
| 4 | LYS | LEU | SER | GLU | LEU | ASP | ASP | ARG | ALA | ASP | ||||
| 5 | ALA | LEU | GLN | ALA | GLY | ALA | SER | GLN | PHE | GLU | ||||
| 6 | THR | SER | ALA | ALA | LYS | LEU | LYS | ARG | LYS | TYR | ||||
| 7 | TRP | TRP | LYS | ASN | LEU |
Entity 2, Syntaxin 1A(191-253) 63 residues - 7541 Da.
| 1 | ALA | LEU | SER | GLU | ILE | GLU | THR | ARG | HIS | SER | ||||
| 2 | GLU | ILE | ILE | LYS | LEU | GLU | ASN | SER | ILE | ARG | ||||
| 3 | GLU | LEU | HIS | ASP | MET | PHE | MET | ASP | MET | ALA | ||||
| 4 | MET | LEU | VAL | GLU | SER | GLN | GLY | GLU | MET | ILE | ||||
| 5 | ASP | ARG | ILE | GLU | TYR | ASN | VAL | GLU | HIS | ALA | ||||
| 6 | VAL | ASP | TYR | VAL | GLU | ARG | ALA | VAL | SER | ASP | ||||
| 7 | THR | LYS | LYS |
Entity 3, SNAP-25B (11-82-W) 75 residues - 8688 Da.
| 1 | GLY | SER | LEU | GLU | GLU | MET | GLN | ARG | ARG | ALA | ||||
| 2 | ASP | GLN | LEU | ALA | ASP | GLU | SER | LEU | GLU | SER | ||||
| 3 | THR | ARG | ARG | MET | LEU | GLN | LEU | VAL | GLU | GLU | ||||
| 4 | SER | LYS | ASP | ALA | GLY | ILE | ARG | THR | LEU | VAL | ||||
| 5 | MET | LEU | ASP | GLU | GLN | GLY | GLU | GLN | LEU | ASP | ||||
| 6 | ARG | VAL | GLU | GLU | GLY | MET | ASN | HIS | ILE | ASN | ||||
| 7 | GLN | ASP | MET | LYS | GLU | ALA | GLU | LYS | ASN | LEU | ||||
| 8 | LYS | ASP | LEU | GLY | TRP |
Entity 4, SNAP-25B (141-203-W) 66 residues - 7603 Da.
| 1 | GLY | SER | ALA | ARG | GLU | ASN | GLU | MET | ASP | GLU | ||||
| 2 | ASN | LEU | GLU | GLN | VAL | SER | GLY | ILE | ILE | GLY | ||||
| 3 | ASN | LEU | ARG | HIS | MET | ALA | LEU | ASP | MET | GLY | ||||
| 4 | ASN | GLU | ILE | ASP | THR | GLN | ASN | ARG | GLN | ILE | ||||
| 5 | ASP | ARG | ILE | MET | GLU | LYS | ALA | ASP | SER | ASN | ||||
| 6 | LYS | THR | ARG | ILE | ASP | GLU | ALA | ASN | GLN | ARG | ||||
| 7 | ALA | THR | LYS | MET | LEU | TRP |
Entity 5, Complexin I(26-83) 56 residues - 7080 Da.
| 1 | PRO | ASP | ALA | ALA | LYS | LYS | GLU | GLU | GLU | ARG | ||||
| 2 | GLN | GLU | ALA | LEU | ARG | GLN | ALA | GLU | GLU | GLU | ||||
| 3 | ARG | LYS | ALA | LYS | TYR | ALA | LYS | MET | GLU | ALA | ||||
| 4 | GLU | ARG | GLU | VAL | MET | ARG | GLN | GLY | ILE | ARG | ||||
| 5 | ASP | LYS | TYR | GLY | ILE | LYS | LYS | LYS | GLU | GLU | ||||
| 6 | ARG | GLU | ALA | GLU | ALA | GLN |
Samples:
sample_1: Synaptobrevin2 SNARE motif, [U-95% 13C; U-90% 15N], 300 mM; Syntaxin 1A SNARE motif 300 mM; SNAP-25B N-terminal SNARE motif 300 mM; SNAP-25B C-terminal SNARE motif 300 mM; Complexin I (26-83) 300 mM
sample_2: Synaptobrevin2 SNARE motif 300 mM; Syntaxin 1A SNARE motif, [U-95% 13C; U-90% 15N], 300 mM; SNAP-25B N-terminal SNARE motif 300 mM; SNAP-25B C-terminal SNARE motif 300 mM; Complexin I (26-83) 300 mM
sample_3: Synaptobrevin2 SNARE motif 300 mM; Syntaxin 1A SNARE motif 300 mM; SNAP-25B N-terminal SNARE motif 300 mM; SNAP-25B C-terminal SNARE motif 300 mM; Complexin I (26-83), [U-95% 13C; U-90% 15N], 300 mM
Ex-cond_1: pH: 7.5; temperature: 305 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| Transverse-relaxation optimized spectroscopy (TROSY)-based triple resonance | not available | not available | Ex-cond_1 |
| experiments were used for assignments. | not available | not available | Ex-cond_1 |
Software:
NMRview v4.12 - Analysis NMR spectra
NMR spectrometers:
- Varian INOVA 600 MHz