BMRB Entry 6281

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6281

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for the Complexin/SNARE complex
Deposition date:
2004-07-30
Original release date:
2004-11-29
Authors:
Chen, Xiaocheng; Rizo, Josep
Citation:

Citation: Chen, Xiaocheng; Tomchick, Diana; Kovrigin, Evguenii; Arac, Demet; Machius, Mischa; Sudhof, Thomas; Rizo, Josep. "Three-Dimensional Structure of the Complexin/SNARE Complex"  Neuron 33, 397-409 (2002).
PubMed: 11832227

Assembly members:

Assembly members:
Synaptobrevin2 SNARE motif, polymer, 65 residues, 7761 Da.
Syntaxin 1A SNARE motif, polymer, 63 residues, 7541 Da.
SNAP-25B N-terminal SNARE motif, polymer, 75 residues, 8688 Da.
SNAP-25B C-terminal SNARE motif, polymer, 66 residues, 7603 Da.
Complexin I (26-83), polymer, 56 residues, 7080 Da.

Natural source:

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Synaptobrevin2 SNARE motif: NRRLQQTQAQVDEVVDIMRV NVDKVLERDQKLSELDDRAD ALQAGASQFETSAAKLKRKY WWKNL
Syntaxin 1A SNARE motif: ALSEIETRHSEIIKLENSIR ELHDMFMDMAMLVESQGEMI DRIEYNVEHAVDYVERAVSD TKK
SNAP-25B N-terminal SNARE motif: GSLEEMQRRADQLADESLES TRRMLQLVEESKDAGIRTLV MLDEQGEQLDRVEEGMNHIN QDMKEAEKNLKDLGW
SNAP-25B C-terminal SNARE motif: GSARENEMDENLEQVSGIIG NLRHMALDMGNEIDTQNRQI DRIMEKADSNKTRIDEANQR ATKMLW
Complexin I (26-83): PDAAKKEEERQEALRQAEEE RKAKYAKMEAEREVMRQGIR DKYGIKKKEEREAEAQ

Data sets:
Data typeCount
1H chemical shifts151
13C chemical shifts153
15N chemical shifts151

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Synaptobrevin2 (29-93)1
2Syntaxin 1A(191-253)2
3SNAP-25B (11-82-W)3
4SNAP-25B (141-203-W)4
5Complexin I(26-83)5

Entities:

Entity 1, Synaptobrevin2 (29-93) 65 residues - 7761 Da.

1   ASNARGARGLEUGLNGLNTHRGLNALAGLN
2   VALASPGLUVALVALASPILEMETARGVAL
3   ASNVALASPLYSVALLEUGLUARGASPGLN
4   LYSLEUSERGLULEUASPASPARGALAASP
5   ALALEUGLNALAGLYALASERGLNPHEGLU
6   THRSERALAALALYSLEULYSARGLYSTYR
7   TRPTRPLYSASNLEU

Entity 2, Syntaxin 1A(191-253) 63 residues - 7541 Da.

1   ALALEUSERGLUILEGLUTHRARGHISSER
2   GLUILEILELYSLEUGLUASNSERILEARG
3   GLULEUHISASPMETPHEMETASPMETALA
4   METLEUVALGLUSERGLNGLYGLUMETILE
5   ASPARGILEGLUTYRASNVALGLUHISALA
6   VALASPTYRVALGLUARGALAVALSERASP
7   THRLYSLYS

Entity 3, SNAP-25B (11-82-W) 75 residues - 8688 Da.

1   GLYSERLEUGLUGLUMETGLNARGARGALA
2   ASPGLNLEUALAASPGLUSERLEUGLUSER
3   THRARGARGMETLEUGLNLEUVALGLUGLU
4   SERLYSASPALAGLYILEARGTHRLEUVAL
5   METLEUASPGLUGLNGLYGLUGLNLEUASP
6   ARGVALGLUGLUGLYMETASNHISILEASN
7   GLNASPMETLYSGLUALAGLULYSASNLEU
8   LYSASPLEUGLYTRP

Entity 4, SNAP-25B (141-203-W) 66 residues - 7603 Da.

1   GLYSERALAARGGLUASNGLUMETASPGLU
2   ASNLEUGLUGLNVALSERGLYILEILEGLY
3   ASNLEUARGHISMETALALEUASPMETGLY
4   ASNGLUILEASPTHRGLNASNARGGLNILE
5   ASPARGILEMETGLULYSALAASPSERASN
6   LYSTHRARGILEASPGLUALAASNGLNARG
7   ALATHRLYSMETLEUTRP

Entity 5, Complexin I(26-83) 56 residues - 7080 Da.

1   PROASPALAALALYSLYSGLUGLUGLUARG
2   GLNGLUALALEUARGGLNALAGLUGLUGLU
3   ARGLYSALALYSTYRALALYSMETGLUALA
4   GLUARGGLUVALMETARGGLNGLYILEARG
5   ASPLYSTYRGLYILELYSLYSLYSGLUGLU
6   ARGGLUALAGLUALAGLN

Samples:

sample_1: Synaptobrevin2 SNARE motif, [U-95% 13C; U-90% 15N], 300 mM; Syntaxin 1A SNARE motif 300 mM; SNAP-25B N-terminal SNARE motif 300 mM; SNAP-25B C-terminal SNARE motif 300 mM; Complexin I (26-83) 300 mM

sample_2: Synaptobrevin2 SNARE motif 300 mM; Syntaxin 1A SNARE motif, [U-95% 13C; U-90% 15N], 300 mM; SNAP-25B N-terminal SNARE motif 300 mM; SNAP-25B C-terminal SNARE motif 300 mM; Complexin I (26-83) 300 mM

sample_3: Synaptobrevin2 SNARE motif 300 mM; Syntaxin 1A SNARE motif 300 mM; SNAP-25B N-terminal SNARE motif 300 mM; SNAP-25B C-terminal SNARE motif 300 mM; Complexin I (26-83), [U-95% 13C; U-90% 15N], 300 mM

Ex-cond_1: pH: 7.5; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
Transverse-relaxation optimized spectroscopy (TROSY)-based triple resonancenot availablenot availableEx-cond_1
experiments were used for assignments.not availablenot availableEx-cond_1

Software:

NMRview v4.12 - Analysis NMR spectra

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA07605 BAA11097 BAE01944 BAE22299 BAE34390 BAG36694 BAA07151 BAA28865 BAA01231 BAA02089 BAF82336 BAA20151 BAB16738 BAC37105 BAA20152 BAA22370 BAA20151 BAE34390 BAE22299 BAE01944 BAA11097 BAA07605
GB AAC52270 AAH14803 AAH93605 ACH43703 EDL20107
REF NP_001232223 NP_001244613 NP_001271686 NP_031782 NP_074055 NP_446240 NP_004594 NP_058081 NP_001028037 NP_001077267 XP_849677 XP_001513821 XP_001493177 XP_001374457 NP_003072 NP_035558 NP_001069714 NP_003072 NP_001009094 NP_001028036 XP_001377543 XP_001094482 XP_001094242 NP_074055 NP_031782
SP P63040 P63041 Q4R4N1
SWISS-PROT Q16623 Q5R4L2 P32850 P32851 O35526 P60879 P60880 P60877 P60878 P36977 Q4R4N1 P63041 P63040
PIR G01485 S38308 S38308 S38309
PRF 2116295A 1604365A 2016338A
GenBank AAB22525 AAF64478 AAA42195 AAA53519 AAA20940 AAV38489 AAH10647 AAC37545 AAA99825 AAA49070 AAA49072 AAA61741 AAA49070 AAA49071 AAA16537 EDL84015 EDL20107 AAH93605 AAH14803 AAC52270
EMBL CAH93304 CAG33299 CAL38529 CAL38108 CAI29660 CAH92726 CAB42860 CAH69032 CAF04071 CAH69031 CAB42860 CAC34534
BMRB 6235 6235 6235
AlphaFold P63040 P63041 Q4R4N1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks