BMRB Entry 6235

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6235
MolProbity Validation Chart

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for the SNARE complex
Deposition date:
2004-06-11
Original release date:
2005-03-07
Authors:
Chen, Xiaocheng; Rizo, Josep
Citation:

Citation: Chen, Xiaocheng; Tang, J.; Sudhof, T.; Rizo, Josep. "Aare Neuronal SNARE Proteins Ca(2+) Sensors?"  J. Mol. Biol. 347, 145-158 (2005).
PubMed: 15733924

Assembly members:

Assembly members:
Synaptobrevin2 SNARE motif, polymer, 65 residues, 7761 Da.
Syntaxin 1A SNARE motif, polymer, 63 residues, 7541 Da.
SNAP-25B N-terminal SNARE motif, polymer, 73 residues, 8688 Da.
SNAP-25B C-terminal SNARE motif, polymer, 64 residues, 7603 Da.

Natural source:

Natural source:   Common Name: 10116   Taxonomy ID: Norway rat   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus Norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Synaptobrevin2 SNARE motif: NRRLQQTQAQVDEVVDIMRV NVDKVLERDQKLSELDDRAD ALQAGASQFETSAAKLKRKY WWKNL
Syntaxin 1A SNARE motif: ALSEIETRHSEIIKLENSIR ELHDMFMDMAMLVESQGEMI DRIEYNVEHAVDYVERAVSD TKK
SNAP-25B N-terminal SNARE motif: LEEMQRRADQLADESLESTR RMLQLVEESKDAGIRTLVML DEQGEQLDRVEEGMNHINQD MKEAEKNLKDLGW
SNAP-25B C-terminal SNARE motif: ARENEMDENLEQVSGIIGNL RHMALDMGNEIDTQNRQIDR IMEKADSNKTRIDEANQRAT KMLW

Data typeCount
1H chemical shifts235
13C chemical shifts238
15N chemical shifts235

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Syb1
2Syx2
3SNN3
4SNC4

Entities:

Entity 1, Syb 65 residues - 7761 Da.

1   ASNARGARGLEUGLNGLNTHRGLNALAGLN
2   VALASPGLUVALVALASPILEMETARGVAL
3   ASNVALASPLYSVALLEUGLUARGASPGLN
4   LYSLEUSERGLULEUASPASPARGALAASP
5   ALALEUGLNALAGLYALASERGLNPHEGLU
6   THRSERALAALALYSLEULYSARGLYSTYR
7   TRPTRPLYSASNLEU

Entity 2, Syx 63 residues - 7541 Da.

1   ALALEUSERGLUILEGLUTHRARGHISSER
2   GLUILEILELYSLEUGLUASNSERILEARG
3   GLULEUHISASPMETPHEMETASPMETALA
4   METLEUVALGLUSERGLNGLYGLUMETILE
5   ASPARGILEGLUTYRASNVALGLUHISALA
6   VALASPTYRVALGLUARGALAVALSERASP
7   THRLYSLYS

Entity 3, SNN 73 residues - 8688 Da.

1   LEUGLUGLUMETGLNARGARGALAASPGLN
2   LEUALAASPGLUSERLEUGLUSERTHRARG
3   ARGMETLEUGLNLEUVALGLUGLUSERLYS
4   ASPALAGLYILEARGTHRLEUVALMETLEU
5   ASPGLUGLNGLYGLUGLNLEUASPARGVAL
6   GLUGLUGLYMETASNHISILEASNGLNASP
7   METLYSGLUALAGLULYSASNLEULYSASP
8   LEUGLYTRP

Entity 4, SNC 64 residues - 7603 Da.

1   ALAARGGLUASNGLUMETASPGLUASNLEU
2   GLUGLNVALSERGLYILEILEGLYASNLEU
3   ARGHISMETALALEUASPMETGLYASNGLU
4   ILEASPTHRGLNASNARGGLNILEASPARG
5   ILEMETGLULYSALAASPSERASNLYSTHR
6   ARGILEASPGLUALAASNGLNARGALATHR
7   LYSMETLEUTRP

Samples:

sample_1: Synaptobrevin2 SNARE motif, [U-2H; U-13C; U-15N], 0.3 mM; Syntaxin 1A SNARE motif 0.3 mM; SNAP-25B N-terminal SNARE motif 0.3 mM; SNAP-25B C-terminal SNARE motif 0.3 mM

sample_2: Synaptobrevin2 SNARE motif 0.3 mM; Syntaxin 1A SNARE motif, [U-2H; U-13C; U-15N], 0.3 mM; SNAP-25B N-terminal SNARE motif 0.3 mM; SNAP-25B C-terminal SNARE motif 0.3 mM

sample_3: Synaptobrevin2 SNARE motif 0.3 mM; Syntaxin 1A SNARE motif 0.3 mM; SNAP-25B N-terminal SNARE motif, [U-2H; U-13C; U-15N], 0.3 mM; SNAP-25B C-terminal SNARE motif 0.3 mM

sample_4: Synaptobrevin2 SNARE motif 0.3 mM; Syntaxin 1A SNARE motif 0.3 mM; SNAP-25B N-terminal SNARE motif 0.3 mM; SNAP-25B C-terminal SNARE motif, [U-2H; U-13C; U-15N], 0.3 mM

Ex-cond_1: pH: 7.5; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
Transverse-relaxation optimized spectroscopy (TROSY) based HNCA and TROSY based HN(CO)CAnot availablenot availablenot available

Software:

NMRview v4.12 -

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA20151 BAA20152 BAA22370 BAB16738 BAC37105 BAG36694 BAA07151 BAA28865 BAA01231 BAA02089 BAF82336 BAA20151 BAC37105 BAB16738 BAA22370 BAA20152 BAA20151
EMBL CAF04071 CAG00145 CAH92513 CAH92726 CAH93161 CAH93304 CAG33299 CAL38529 CAL38108 CAI29660 CAH92726 CAB42860 CAH69032 CAH69031 CAF04071 CAC34534 CAB42860
GB AAA16537 AAA49070 AAA49071 AAA49072 AAA61741
PIR S38308 S38309 G01485 S38308 S38309 S38308
PRF 1604365A 2016338A 2116295A 2016338A 1604365A
REF NP_001009094 NP_001028036 NP_001069714 NP_001127109 NP_001128960 NP_446240 NP_004594 NP_058081 NP_001028037 NP_001077267 XP_849677 XP_001513821 XP_001493177 XP_001374457 NP_003072 NP_035558 NP_003072 NP_001069714 NP_001028036 NP_001009094
SP P36977 P60877 P60878 P60879 P60880
TPG DAA23666
SWISS-PROT Q16623 Q5R4L2 P32850 P32851 O35526 P60880 P60879 P60878 P60877 P36977
GenBank AAB22525 AAF64478 AAA42195 AAA53519 AAA20940 AAV38489 AAH10647 AAC37545 AAA99825 AAA49070 AAA61741 AAA49072 AAA49071 AAA49070 AAA16537
BMRB 6281 6281 6281
AlphaFold P36977 P60877 P60878 P60879 P60880

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks