BMRB Entry 6235
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6235
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Chen, Xiaocheng; Tang, J.; Sudhof, T.; Rizo, Josep. "Aare Neuronal SNARE Proteins Ca(2+) Sensors?" J. Mol. Biol. 347, 145-158 (2005).
PubMed: 15733924
Assembly members:
Synaptobrevin2 SNARE motif, polymer, 65 residues, 7761 Da.
Syntaxin 1A SNARE motif, polymer, 63 residues, 7541 Da.
SNAP-25B N-terminal SNARE motif, polymer, 73 residues, 8688 Da.
SNAP-25B C-terminal SNARE motif, polymer, 64 residues, 7603 Da.
Natural source: Common Name: 10116 Taxonomy ID: Norway rat Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus Norvegicus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Synaptobrevin2 SNARE motif: NRRLQQTQAQVDEVVDIMRV
NVDKVLERDQKLSELDDRAD
ALQAGASQFETSAAKLKRKY
WWKNL
Syntaxin 1A SNARE motif: ALSEIETRHSEIIKLENSIR
ELHDMFMDMAMLVESQGEMI
DRIEYNVEHAVDYVERAVSD
TKK
SNAP-25B N-terminal SNARE motif: LEEMQRRADQLADESLESTR
RMLQLVEESKDAGIRTLVML
DEQGEQLDRVEEGMNHINQD
MKEAEKNLKDLGW
SNAP-25B C-terminal SNARE motif: ARENEMDENLEQVSGIIGNL
RHMALDMGNEIDTQNRQIDR
IMEKADSNKTRIDEANQRAT
KMLW
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 235 |
| 13C chemical shifts | 238 |
| 15N chemical shifts | 235 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Syb | 1 |
| 2 | Syx | 2 |
| 3 | SNN | 3 |
| 4 | SNC | 4 |
Entities:
Entity 1, Syb 65 residues - 7761 Da.
| 1 | ASN | ARG | ARG | LEU | GLN | GLN | THR | GLN | ALA | GLN | ||||
| 2 | VAL | ASP | GLU | VAL | VAL | ASP | ILE | MET | ARG | VAL | ||||
| 3 | ASN | VAL | ASP | LYS | VAL | LEU | GLU | ARG | ASP | GLN | ||||
| 4 | LYS | LEU | SER | GLU | LEU | ASP | ASP | ARG | ALA | ASP | ||||
| 5 | ALA | LEU | GLN | ALA | GLY | ALA | SER | GLN | PHE | GLU | ||||
| 6 | THR | SER | ALA | ALA | LYS | LEU | LYS | ARG | LYS | TYR | ||||
| 7 | TRP | TRP | LYS | ASN | LEU |
Entity 2, Syx 63 residues - 7541 Da.
| 1 | ALA | LEU | SER | GLU | ILE | GLU | THR | ARG | HIS | SER | ||||
| 2 | GLU | ILE | ILE | LYS | LEU | GLU | ASN | SER | ILE | ARG | ||||
| 3 | GLU | LEU | HIS | ASP | MET | PHE | MET | ASP | MET | ALA | ||||
| 4 | MET | LEU | VAL | GLU | SER | GLN | GLY | GLU | MET | ILE | ||||
| 5 | ASP | ARG | ILE | GLU | TYR | ASN | VAL | GLU | HIS | ALA | ||||
| 6 | VAL | ASP | TYR | VAL | GLU | ARG | ALA | VAL | SER | ASP | ||||
| 7 | THR | LYS | LYS |
Entity 3, SNN 73 residues - 8688 Da.
| 1 | LEU | GLU | GLU | MET | GLN | ARG | ARG | ALA | ASP | GLN | ||||
| 2 | LEU | ALA | ASP | GLU | SER | LEU | GLU | SER | THR | ARG | ||||
| 3 | ARG | MET | LEU | GLN | LEU | VAL | GLU | GLU | SER | LYS | ||||
| 4 | ASP | ALA | GLY | ILE | ARG | THR | LEU | VAL | MET | LEU | ||||
| 5 | ASP | GLU | GLN | GLY | GLU | GLN | LEU | ASP | ARG | VAL | ||||
| 6 | GLU | GLU | GLY | MET | ASN | HIS | ILE | ASN | GLN | ASP | ||||
| 7 | MET | LYS | GLU | ALA | GLU | LYS | ASN | LEU | LYS | ASP | ||||
| 8 | LEU | GLY | TRP |
Entity 4, SNC 64 residues - 7603 Da.
| 1 | ALA | ARG | GLU | ASN | GLU | MET | ASP | GLU | ASN | LEU | ||||
| 2 | GLU | GLN | VAL | SER | GLY | ILE | ILE | GLY | ASN | LEU | ||||
| 3 | ARG | HIS | MET | ALA | LEU | ASP | MET | GLY | ASN | GLU | ||||
| 4 | ILE | ASP | THR | GLN | ASN | ARG | GLN | ILE | ASP | ARG | ||||
| 5 | ILE | MET | GLU | LYS | ALA | ASP | SER | ASN | LYS | THR | ||||
| 6 | ARG | ILE | ASP | GLU | ALA | ASN | GLN | ARG | ALA | THR | ||||
| 7 | LYS | MET | LEU | TRP |
Samples:
sample_1: Synaptobrevin2 SNARE motif, [U-2H; U-13C; U-15N], 0.3 mM; Syntaxin 1A SNARE motif 0.3 mM; SNAP-25B N-terminal SNARE motif 0.3 mM; SNAP-25B C-terminal SNARE motif 0.3 mM
sample_2: Synaptobrevin2 SNARE motif 0.3 mM; Syntaxin 1A SNARE motif, [U-2H; U-13C; U-15N], 0.3 mM; SNAP-25B N-terminal SNARE motif 0.3 mM; SNAP-25B C-terminal SNARE motif 0.3 mM
sample_3: Synaptobrevin2 SNARE motif 0.3 mM; Syntaxin 1A SNARE motif 0.3 mM; SNAP-25B N-terminal SNARE motif, [U-2H; U-13C; U-15N], 0.3 mM; SNAP-25B C-terminal SNARE motif 0.3 mM
sample_4: Synaptobrevin2 SNARE motif 0.3 mM; Syntaxin 1A SNARE motif 0.3 mM; SNAP-25B N-terminal SNARE motif 0.3 mM; SNAP-25B C-terminal SNARE motif, [U-2H; U-13C; U-15N], 0.3 mM
Ex-cond_1: pH: 7.5; temperature: 305 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | not available | not available | not available |
| Transverse-relaxation optimized spectroscopy (TROSY) based HNCA and TROSY based HN(CO)CA | not available | not available | not available |
Software:
NMRview v4.12 -
NMR spectrometers:
- Varian INOVA 600 MHz