BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6202

Title: Assignment of the 1H, 15N and 13C resonances of the Class II E2 Conjugating Enzyme, Ubc1

Deposition date: 2004-05-07 Original release date: 2004-12-16

Authors: Merkley, Nadine; Shaw, Gary

Citation: Merkley, Nadine; Shaw, Gary. "Letter to the Editor: Assignment of the 1H, 13C, and 15N resonances of the class II E2 conjugating enzyme, Ubc1"  J. Biomol NMR 30, 369-370 (2004).

Assembly members:
yeast Ubc1, polymer, 215 residues, 24162 Da.

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
yeast Ubc1: SSRAKRIMKEIQAVKDDPAA HITLEFVSESDIHHLKGTFL GPPGTPYEGGKFVVDIEVPM EYPFKPPKMQFDTKVYHPNI SSVTGAICLDILRNAWSPVI TLKSALISLQALLQSPEPND PQDAEVAQHYLRDRESFNKT AALWTRLYASETSNGQKGNV EESDLYGIDHDLIDEFESQG FEKDKIVEVLRRLGVKSLDP NDNNTANRIIEELLK

Data sets:
Data typeCount
1H chemical shifts1307
13C chemical shifts804
15N chemical shifts190

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1yUbc1(K93R)1

Entities:

Entity 1, yUbc1(K93R) 215 residues - 24162 Da.

1   SERSERARGALALYSARGILEMETLYSGLU
2   ILEGLNALAVALLYSASPASPPROALAALA
3   HISILETHRLEUGLUPHEVALSERGLUSER
4   ASPILEHISHISLEULYSGLYTHRPHELEU
5   GLYPROPROGLYTHRPROTYRGLUGLYGLY
6   LYSPHEVALVALASPILEGLUVALPROMET
7   GLUTYRPROPHELYSPROPROLYSMETGLN
8   PHEASPTHRLYSVALTYRHISPROASNILE
9   SERSERVALTHRGLYALAILECYSLEUASP
10   ILELEUARGASNALATRPSERPROVALILE
11   THRLEULYSSERALALEUILESERLEUGLN
12   ALALEULEUGLNSERPROGLUPROASNASP
13   PROGLNASPALAGLUVALALAGLNHISTYR
14   LEUARGASPARGGLUSERPHEASNLYSTHR
15   ALAALALEUTRPTHRARGLEUTYRALASER
16   GLUTHRSERASNGLYGLNLYSGLYASNVAL
17   GLUGLUSERASPLEUTYRGLYILEASPHIS
18   ASPLEUILEASPGLUPHEGLUSERGLNGLY
19   PHEGLULYSASPLYSILEVALGLUVALLEU
20   ARGARGLEUGLYVALLYSSERLEUASPPRO
21   ASNASPASNASNTHRALAASNARGILEILE
22   GLUGLULEULEULYS

Samples:

sample_1: yeast Ubc1, [U-15N; U-13C], 0.48 mM; Tris 25 mM; EDTA 1 mM; DTT 1 mM; NaCl 150 mM

Ex-cond_1: pH: 7.5; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
HC(CO)NHnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available

Software:

NMRPipe -

Pipp -

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 4567
PDB
DBJ GAA22407
EMBL CAA39812 CAA86682 CAY78679
GB AAS56001 AHY75163 EDN60515 EDV08135 EDZ73074
REF NP_010462
SP P21734
TPG DAA12019

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts