BMRB Entry 4567

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4567

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C resonance assignments for the catalytic domain of the yeast E2, UBC1

Deposition date:

1999-12-22

Original release date:

2000-04-03

Authors:

Hamilton, Katherine; Ellison, Michael; Shaw, Gary

Citation:

Citation: Hamilton, Katherine; Ellison, Michael; Shaw, Gary. "Letter to the Editor: 1H, 15N and 13C Resonance Assignments for the Catalytic Domain of the Yeast E2, UBC1" J. Biomol. NMR 16, 351-352 (2000).

Assembly members:

Assembly members:
yeast UBC1 (catalytic domain), polymer, 151 residues, 16821 Da.

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: not available Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Vector: pET3A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
yeast UBC1 (catalytic domain): MSSRAKRIMKEIQAVKDDPA AHITLEFVSESDIHHLKGTF LGPPGTPYEGGKFVVDIEVP MEYPFKPPKMQFDTKVYHPN ISSVTGAICLDILRNAWSPV ITLKSALISLQALLQSPEPN DPQDAEVAQHYLRDRESFNK TAALWTRLYAS

Data sets:

assigned_chemical_shifts
- chemical_shifts

Data type	Count
1H chemical shifts	576
13C chemical shifts	463
15N chemical shifts	130

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	yUBC1D450(K93R)	1

Entities:

Entity 1, yUBC1D450(K93R) 151 residues - 16821 Da.

1

MET

SER

ARG

ALA

LYS

ARG

ILE

MET

LYS

2

GLU

ILE

GLN

ALA

VAL

LYS

ASP

PRO

ALA

3

ALA

HIS

ILE

THR

LEU

GLU

PHE

VAL

SER

GLU

4

SER

ASP

ILE

HIS

LEU

LYS

GLY

THR

PHE

5

LEU

GLY

PRO

GLY

THR

PRO

TYR

GLU

GLY

6

GLY

LYS

PHE

VAL

ASP

ILE

GLU

VAL

PRO

7

MET

GLU

TYR

PRO

PHE

LYS

PRO

LYS

MET

8

GLN

PHE

ASP

THR

LYS

VAL

TYR

HIS

PRO

ASN

9

ILE

SER

VAL

THR

GLY

ALA

ILE

CYS

LEU

10

ASP

ILE

LEU

ARG

ASN

ALA

TRP

SER

PRO

VAL

11

ILE

THR

LEU

LYS

SER

ALA

LEU

ILE

SER

LEU

12

GLN

ALA

LEU

GLN

SER

PRO

GLU

PRO

ASN

13

ASP

PRO

GLN

ASP

ALA

GLU

VAL

ALA

GLN

HIS

14

TYR

LEU

ARG

ASP

ARG

GLU

SER

PHE

ASN

LYS

15

THR

ALA

LEU

TRP

THR

ARG

LEU

TYR

ALA

16

SER

Samples:

sample_1: yeast UBC1 (catalytic domain), [U-95% 13C; U-95% 15N], 0.7 mM; HEPES 40 mM; NaCl 400 mM; DTT 1.0 mM

sample_2: yeast UBC1 (catalytic domain), [U-95% 15N], 0.8 – 0.9 mM; HEPES 40 mM; NaCl 400 mM; DTT 1.0 mM

conditions: pH: 7.5 na; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	not available
15N-edited NOESY	not available	not available	not available
15N-edited TOCSY	not available	not available	not available
HNCACB	not available	not available	not available
CBCACONH	not available	not available	not available
HCCH-TOCSY	not available	not available	not available
HNCO	not available	not available	not available
HCACOCANH	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Varian Unity 500 MHz
Varian Inova 600 MHz
Varian Inova 800 MHz

PDB	1FXT 1FZY 1TTE
DBJ	GAA22407
EMBL	CAA39812 CAA86682 CAY78679
GB	AAS56001 AHY75163 AJP37891 AJU58013 AJU58708
REF	NP_010462
SP	P21734
TPG	DAA12019
AlphaFold	P21734