BMRB Entry 6196

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6196

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for Arath;CDC25; a dual-specificity tyrosine phosphatase from Arabidopsis thaliana
Deposition date:
2004-04-26
Original release date:
2004-09-07
Authors:
Landrieu, Isabelle; Wieruszeski, Jean-Michel; Hassan, Sahar; Lippens, Guy
Citation:

Citation: Landrieu, Isabelle; Da Costa, M.; De Veylder, L.; Dewitte, F.; Vandepoele, K.; Hassan, Sahar; Wieruszeski, Jean-Michel; Faure, J.; Von Montagu, M.; Inze, D.; Lippens, Guy. "A small CDC25 dual-specificity tyrosine-phosphatase isoform in Arabidopsis thaliana"  Proc. Natl. Acad. Sci. U.S.A. ., .-. (2004).
PubMed: 15329414

Assembly members:

Assembly members:
Dual-specificity tyrosine phosphatase; CYS131SER mutant, polymer, 146 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Dual-specificity tyrosine phosphatase; CYS131SER mutant: MGRSIFSFFTKKKKMAMARS ISYITSTQLLPLHRRPNIAI IDVRDEERNYDGHIAGSLHY ASGSFDDKISHLVQNVKDKD TLVFHSALSQVRGPTCARRL VNYLDEKKEDTGIKNIMILE RGFNGWEASGKPVCRCAEVP CKGDSA

Data sets:
Data typeCount
13C chemical shifts388
15N chemical shifts124
1H chemical shifts583

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Arath; CDC25 monomer; CYS131SER mutant1
2ZINC (II) ION2

Entities:

Entity 1, Arath; CDC25 monomer; CYS131SER mutant 146 residues - Formula weight is not available

1   METGLYARGSERILEPHESERPHEPHETHR
2   LYSLYSLYSLYSMETALAMETALAARGSER
3   ILESERTYRILETHRSERTHRGLNLEULEU
4   PROLEUHISARGARGPROASNILEALAILE
5   ILEASPVALARGASPGLUGLUARGASNTYR
6   ASPGLYHISILEALAGLYSERLEUHISTYR
7   ALASERGLYSERPHEASPASPLYSILESER
8   HISLEUVALGLNASNVALLYSASPLYSASP
9   THRLEUVALPHEHISSERALALEUSERGLN
10   VALARGGLYPROTHRCYSALAARGARGLEU
11   VALASNTYRLEUASPGLULYSLYSGLUASP
12   THRGLYILELYSASNILEMETILELEUGLU
13   ARGGLYPHEASNGLYTRPGLUALASERGLY
14   LYSPROVALCYSARGCYSALAGLUVALPRO
15   CYSLYSGLYASPSERALA

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Dual-specificity tyrosine phosphatase; CYS131SER mutant, [U-90% 15N], 400 uM

sample_2: Dual-specificity tyrosine phosphatase; CYS131SER mutant, [U-95% 13C; U-90% 15N], 400 uM

sample_3: Dual-specificity tyrosine phosphatase; CYS131SER mutant, [U-95% 13C], 400 uM

sample_4: Dual-specificity tyrosine phosphatase; CYS131SER mutant, [15N]-Lys, 250 uM

cond-1: pH: 7.6; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablecond-1
1H-13C NOESYnot availablenot availablecond-1
HNCAnot availablenot availablecond-1
HNCOnot availablenot availablecond-1
HN(CA)COnot availablenot availablecond-1
CBCANHnot availablenot availablecond-1

Software:

UXNMR - data acquisition

SNARF - data processing, peak picking (in-house development)

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

SWISS-PROT Q8GY31
REF NP_568119
GenBank AAO39886 AAM63780
EMBL CAB83305
DBJ BAC42537
BMRB 6195

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks