BMRB Entry 6195

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Arath;CDC25; a dual-specificity tyrosine phosphatase from Arabidopsis thaliana
Published: 2004-09-07

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6195

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Arath;CDC25; a dual-specificity tyrosine phosphatase from Arabidopsis thaliana

Deposition date:

2004-04-26

Original release date:

2004-09-07

Authors:

Landrieu, Isabelle; Wieruszeski, Jean-Michel; Hassan, Sahar; Lippens, Guy

Citation:

Citation: Landrieu, Isabelle; Da Costa, M.; De Veylder, L.; Dewitte, F.; Vandepoele, K.; Hassan, Sahar; Wieruszeski, Jean-Michel; Faure, J.; Von Montagu, M.; Inze, D.; Lippens, Guy. "A small CDC25 dual-specificity tyrosine-phosphatase isoform in Arabidopsis thaliana" Proc. Natl. Acad. Sci. U.S.A. ., .-. (2004).
PubMed: 15329414

Assembly members:

Assembly members:
Dual-specificity tyrosine phosphatase, polymer, 132 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Dual-specificity tyrosine phosphatase: MAMARSISYITSTQLLPLHR RPNIAIIDVRDEERNYDGHI AGSLHYASGSFDDKISHLVQ NVKDKDTLVFHSALSQVRGP TCARRLVNYLDEKKEDTGIK NIMILERGFNGWEASGKPVC RCAEVPCKGDCA

Data sets:

assigned_chemical_shifts
- Arath;CDC25C72S_shift_set_1

Data type	Count
1H chemical shifts	627
13C chemical shifts	392
15N chemical shifts	127

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Arath; CDC25 monomer	1
2	ZINC (II) ION	2

Entities:

Entity 1, Arath; CDC25 monomer 132 residues - Formula weight is not available

1

MET

ALA

MET

ALA

ARG

SER

ILE

SER

TYR

ILE

2

THR

SER

THR

GLN

LEU

PRO

LEU

HIS

ARG

3

ARG

PRO

ASN

ILE

ALA

ILE

ASP

VAL

ARG

4

ASP

GLU

ARG

ASN

TYR

ASP

GLY

HIS

ILE

5

ALA

GLY

SER

LEU

HIS

TYR

ALA

SER

GLY

SER

6

PHE

ASP

LYS

ILE

SER

HIS

LEU

VAL

GLN

7

ASN

VAL

LYS

ASP

LYS

ASP

THR

LEU

VAL

PHE

8

HIS

SER

ALA

LEU

SER

GLN

VAL

ARG

GLY

PRO

9

THR

CYS

ALA

ARG

LEU

VAL

ASN

TYR

LEU

10

ASP

GLU

LYS

GLU

ASP

THR

GLY

ILE

LYS

11

ASN

ILE

MET

ILE

LEU

GLU

ARG

GLY

PHE

ASN

12

GLY

TRP

GLU

ALA

SER

GLY

LYS

PRO

VAL

CYS

13

ARG

CYS

ALA

GLU

VAL

PRO

CYS

LYS

GLY

ASP

14

CYS

ALA

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: Dual-specificity tyrosine phosphatase, [U-90% 15N], 400 uM

sample_2: Dual-specificity tyrosine phosphatase, [U-95% 13C; U-90% 15N], 400 uM

sample_3: Dual-specificity tyrosine phosphatase, [U-95% 13C], 400 uM

sample_4: Dual-specificity tyrosine phosphatase, [15N]-Lys, 250 uM

cond-1: pH: 7.6; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY	not available	not available	cond-1
1H-13C NOESY	not available	not available	cond-1
HNCA	not available	not available	cond-1
HNCO	not available	not available	cond-1
HN(CA)CO	not available	not available	cond-1
CBCANH	not available	not available	cond-1

Software:

UXNMR - data acquisition

SNARF - data processing, peak picking (in-house development)

NMR spectrometers:

Bruker DMX 600 MHz
Bruker Avance 800 MHz

REF	NP_568119
SWISS-PROT	Q8GY31
GenBank	AAM63780 AAO39886
DBJ	BAC42537
EMBL	CAB83305
BMRB	6196
PDB	1T3K