BMRB Entry 6192

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6192
MolProbity Validation Chart

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Title:
1H, 13C, and 15N Chemical Shift Assignments for Omega-atracotoxin-Hv1a at pH 3.6
Deposition date:
2004-04-23
Original release date:
2004-06-30
Authors:
Tedford, Hugo; Maciejewski, Mark; King, Glenn
Citation:

Citation: Tedford, Hugo; Maciejewski, Mark; King, Glenn. "1H, 13C, and 15N Chemical Shift Assignments for Omega-atracotoxin-Hv1a at pH 3.6"  .

Assembly members:

Assembly members:
Omega-atracotoxin-Hv1a, polymer, 37 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Blue Mountains Funnel-Web Spider   Taxonomy ID: 6904   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Hadronyche versuta

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Omega-atracotoxin-Hv1a: SPTCIPSGQPCPYNENCCSQ SCTFKENENGNTVKRCD

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts123
15N chemical shifts39
1H chemical shifts215

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Omega-ACTX-Hv1a1

Entities:

Entity 1, Omega-ACTX-Hv1a 37 residues - Formula weight is not available

1   SERPROTHRCYSILEPROSERGLYGLNPRO
2   CYSPROTYRASNGLUASNCYSCYSSERGLN
3   SERCYSTHRPHELYSGLUASNGLUASNGLY
4   ASNTHRVALLYSARGCYSASP

Samples:

sample_1: Omega-atracotoxin-Hv1a, [U-13C; U-15N], 2 – 5 mM; NaCl 50 mM; sodium phosphate 20 mM

sample_2: Omega-atracotoxin-Hv1a, [U-13C; U-15N], 2 – 5 mM; D2O 100%

Ex-cond_1: ionic strength: 0.010 M; pH: 3.6; temperature: 298 K

Ex-cond_2: ionic strength: 0.010 M; pH*: 3.6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESY-HSQCnot availablenot availablenot available
3D 1H-13C HSQC-NOESYnot availablenot availablenot available
2D HNCOnot availablenot availablenot available
2D HNCACOnot availablenot availablenot available
3D (H)CCOHN-TOCSYnot availablenot availablenot available
3D 1H-15N TOCSYnot availablenot availablenot available
3D HNHAnot availablenot availablenot available
3D HNHBnot availablenot availablenot available
2D 1H-1H NOESYnot availablenot availablenot available

Software:

nmrPipe - spectral processing

Xeasy - resonance assignment

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 4233 4937 5702 6194
PDB
EMBL CAI79354
GenBank ABP63656
SWISS-PROT P56207

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks