BMRB Entry 4937

Title:
1H chemical shifts for 'Hairpinless' mutant of omega-atracotoxin-Hv1a
Deposition date:
2001-01-10
Original release date:
2001-01-23
Authors:
Fletcher, Jamie; King, Glenn
Citation:

Citation: Tedford, Hugo; Fletcher, Jamie; King, Glenn. "Functional significance of the beta-hairpin in the insecticidal neurotoxin omega-atracotoxin-Hv1a"  .

Assembly members:

Assembly members:
Hairpinless mutant of omega-atracotoxin-Hv1a, polymer, 25 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 6904   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Hadronyche versuta

Experimental source:   Production method: chemical synthesis

Experimental source:

Natural source:   Common Name: not available   Taxonomy ID: 6904   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Hadronyche versuta

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hairpinless mutant of omega-atracotoxin-Hv1a: CIPSGQPCPYNENCCSQSCT GGRCD

Data sets:
Data typeCount
1H chemical shifts127

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hairpinless monomer1

Entities:

Entity 1, hairpinless monomer 25 residues - Formula weight is not available

1   CYSILEPROSERGLYGLNPROCYSPROTYR
2   ASNGLUASNCYSCYSSERGLNSERCYSTHR
3   GLYGLYARGCYSASP

Samples:

Sample_1: Hairpinless mutant of omega-atracotoxin-Hv1a 3 mM; chloramphenicol 25 uM; TSP 0.1 mM

Ex-cond_1: ionic strength: 0.005 M; pH: 4.9; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYSample_1not availableEx-cond_1
2D 1H-1H TOCSYSample_1not availableEx-cond_1
2D 1H-1H DQF-COSYSample_1not availableEx-cond_1
2D 1H-1H E.COSYSample_1not availableEx-cond_1

Software:

xwinnmr v2.0 - data acquisition, data processing

XEASY v1.3.13 - data acquisition, data processing, peak integration, spectral analysis

NMR spectrometers:

  • Bruker DRX 600 MHz