BMRB Entry 6126

Name: NMR Structure of the CRIB-PDZ module of Par-6
Published: 2004-05-15

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PDB ID: 1ry4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6126
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Structure of the CRIB-PDZ module of Par-6

Deposition date:

2004-03-03

Original release date:

2004-05-15

Authors:

Peterson, F.; R Penkert, R.; Volkman, B.; Prehoda, K.

Citation:

Citation: Peterson, F.; Penkert, R.; Volkman, B.; Prehoda, K.. "Cdc42 Regulates the Par-6 PDZ Domain Through an Allosteric CRIB-PDZ Transition" Mol. Cell 13, 665-676 (2004).
PubMed: 15023337

Assembly members:

Assembly members:
Par-6, polymer, 128 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Par-6: GSKTKAPSISIPHDFRQVSA IIDVDIVPETHRRVRLLKHG SDKPLGFYIRDGTSVRVTAS GLEKQPGIFISRLVPGGLAE STGLLAVNDEVIEVNGIEVA GKTLDQVTDMMVANSSNLII TVKPANQR

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	531
15N chemical shifts	127
1H chemical shifts	831

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Par-6	1

Entities:

Entity 1, Par-6 128 residues - Formula weight is not available

1

GLY

SER

LYS

THR

LYS

ALA

PRO

SER

ILE

SER

2

ILE

PRO

HIS

ASP

PHE

ARG

GLN

VAL

SER

ALA

3

ILE

ASP

VAL

ASP

ILE

VAL

PRO

GLU

THR

4

HIS

ARG

VAL

ARG

LEU

LYS

HIS

GLY

5

SER

ASP

LYS

PRO

LEU

GLY

PHE

TYR

ILE

ARG

6

ASP

GLY

THR

SER

VAL

ARG

VAL

THR

ALA

SER

7

GLY

LEU

GLU

LYS

GLN

PRO

GLY

ILE

PHE

ILE

8

SER

ARG

LEU

VAL

PRO

GLY

LEU

ALA

GLU

9

SER

THR

GLY

LEU

ALA

VAL

ASN

ASP

GLU

10

VAL

ILE

GLU

VAL

ASN

GLY

ILE

GLU

VAL

ALA

11

GLY

LYS

THR

LEU

ASP

GLN

VAL

THR

ASP

MET

12

MET

VAL

ALA

ASN

SER

ASN

LEU

ILE

13

THR

VAL

LYS

PRO

ALA

ASN

GLN

ARG

Samples:

sample_1: Par-6, [U-15N; U-13C], 1 mM; phosphate buffer 20 mM; sodium chloride 50 mM; sodium azide 0.05%; H2O 90%; D2O 10%

sample_2: Par-6, [U-15N], 0.5 mM; phosphate buffer 20 mM; sodium azide 0.05%; sodium chloride 50 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 5.5; temperature: 303 K; ionic strength: 50 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	not available	not available	sample_cond_1
3D 15N-separated NOESY	not available	not available	sample_cond_1
3D 13C-separated aromatic NOESY	not available	not available	sample_cond_1

Software:

XWINNMR v3.1 - collection

NMRPipe v97.027.12.56 - processing

Xeasy v1.3.13 - data analysis

Garant v2.1 - data analysis

Cyana v1.0.6 - structure solution

XPLOR-NIH v2.0.6 - refinement

NMR spectrometers:

Bruker AVANCE 600 MHz

BMRB	17599 17600
PDB	1RY4 1RZX 1X8S 2LC6 2LC7
GB	AAD15927 AAF48757 AAL28917 AAN09592 ACL90845
REF	NP_573238 NP_728094 XP_001355305 XP_001963708 XP_001977590