BMRB Entry 17599

Name: Solution structure of Par-6 Q144C/L164C
Published: 2011-12-01

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PDB ID: 2lc6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17599
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of Par-6 Q144C/L164C

Deposition date:

2011-04-25

Original release date:

2011-12-01

Authors:

Volkman, B.; Whitney, D.; Peterson, F.

Citation:

Citation: Whitney, Dustin; Peterson, Francis; Volkman, Brian. "A Conformational Switch in the CRIB-PDZ Module of Par-6" Structure 19, 1711-1722 (2011).
PubMed: 22078569

Assembly members:

Assembly members:
Par-6, polymer, 128 residues, 13724.863 Da.

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pBH4

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Par-6: GSKTKAPSISIPHDFRCVSA IIDVDIVPETHRRVRLCKHG SDKPLGFYIRDGTSVRVTAS GLEKQPGIFISRLVPGGLAE STGLLAVNDEVIEVNGIEVA GKTLDQVTDMMVANSSNLII TVKPANQR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	529
15N chemical shifts	123
1H chemical shifts	869

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Par-6	1

Entities:

Entity 1, Par-6 128 residues - 13724.863 Da.

The N-terminal GS dipeptide is a cloning artifact.

1

GLY

SER

LYS

THR

LYS

ALA

PRO

SER

ILE

SER

2

ILE

PRO

HIS

ASP

PHE

ARG

CYS

VAL

SER

ALA

3

ILE

ASP

VAL

ASP

ILE

VAL

PRO

GLU

THR

4

HIS

ARG

VAL

ARG

LEU

CYS

LYS

HIS

GLY

5

SER

ASP

LYS

PRO

LEU

GLY

PHE

TYR

ILE

ARG

6

ASP

GLY

THR

SER

VAL

ARG

VAL

THR

ALA

SER

7

GLY

LEU

GLU

LYS

GLN

PRO

GLY

ILE

PHE

ILE

8

SER

ARG

LEU

VAL

PRO

GLY

LEU

ALA

GLU

9

SER

THR

GLY

LEU

ALA

VAL

ASN

ASP

GLU

10

VAL

ILE

GLU

VAL

ASN

GLY

ILE

GLU

VAL

ALA

11

GLY

LYS

THR

LEU

ASP

GLN

VAL

THR

ASP

MET

12

MET

VAL

ALA

ASN

SER

ASN

LEU

ILE

13

THR

VAL

LYS

PRO

ALA

ASN

GLN

ARG

Samples:

sample_1: Par-6, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 53 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D_15N-separated_NOESY	sample_1	isotropic	sample_conditions_1
3D_13C-separated_NOESY	sample_1	isotropic	sample_conditions_1
3D_13C-separated_NOESY (AROMATIC)	sample_1	isotropic	sample_conditions_1

Software:

X-PLOR NIH v2.9.3, SCHWIETERS,C.D.,KUSZEWSKI,J.J.,TJANDRA,N.,CLORE,G.M. - refinement

TOPSPIN v2.1, Bruker - collection

NMRPipe v2007, Delagio,F. et al. - processing

XEASY v1.3, Eccles, C., Guntert, P., Billeter, M., Wuthrich, K. - data analysis

GARANT v2.1, C. Bartels - data analysis

CYANA v2.1, Guntert, P. - structural calculation

NMR spectrometers:

Bruker Avance II 600 MHz

BMRB	17600
PDB	1RY4 1RZX 1X8S 2LC6 2LC7
GB	AAD15927 AAF48757 AAL28917 AAN09592 ACL90845
REF	NP_573238 NP_728094 XP_001963708 XP_001977590 XP_001991396