BMRB Entry 6008

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6008

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
1H, 13C and 15N resonance assignment of the Calcium Bound Form of the C-type Lectin-like Domain of Tetranectin
Deposition date:
2003-11-14
Original release date:
2004-07-08
Authors:
Nielbo, Steen; Thomsen, Jens; Jensen, Peter; Graversen, Jonas; Etzerodt, Michael; Poulsen, Flemming; Thogersen, Hans
Citation:

Citation: Nielbo, Steen; Thomsen, Jens; Jensen, Peter; Graversen, Jonas; Etzerodt, Michael; Poulsen, Flemming; Thogersen, Hans. "Structure of the Plasminogen Kringle 4 Binding Calcium Bound Form of the C-type Lectin-like Domain of Tetranectin"  Biochemistry 43, 8636-8643 (2004).

Assembly members:

Assembly members:
CTLD of tetranectin, polymer, 137 residues, 15122 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CTLD of tetranectin: ALQTVCLKGTKVHMKCFLAF TQTKTFHEASEDCISRGGTL STPQTGSENDALYEYLRQSV GNEAEIWLGLNDMAAEGTWV DMTGARIAYKNWETEITAQP DGGKTENCAVLSGAANGKWF DKRCRDQLPYICQFGIV

Data sets:
Data typeCount
13C chemical shifts323
15N chemical shifts111
1H chemical shifts493

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tn3 with calcium bound1
2calcium ion2

Entities:

Entity 1, tn3 with calcium bound 137 residues - 15122 Da.

1   ALALEUGLNTHRVALCYSLEULYSGLYTHR
2   LYSVALHISMETLYSCYSPHELEUALAPHE
3   THRGLNTHRLYSTHRPHEHISGLUALASER
4   GLUASPCYSILESERARGGLYGLYTHRLEU
5   SERTHRPROGLNTHRGLYSERGLUASNASP
6   ALALEUTYRGLUTYRLEUARGGLNSERVAL
7   GLYASNGLUALAGLUILETRPLEUGLYLEU
8   ASNASPMETALAALAGLUGLYTHRTRPVAL
9   ASPMETTHRGLYALAARGILEALATYRLYS
10   ASNTRPGLUTHRGLUILETHRALAGLNPRO
11   ASPGLYGLYLYSTHRGLUASNCYSALAVAL
12   LEUSERGLYALAALAASNGLYLYSTRPPHE
13   ASPLYSARGCYSARGASPGLNLEUPROTYR
14   ILECYSGLNPHEGLYILEVAL

Entity 2, calcium ion - Ca - 40.078 Da.

1   CA

Samples:

sample_1: CTLD of tetranectin, [U-13C; U-15N], 0.6 mM; CaCl2 2 mM; NaN3 1 mM; D2O 10%

cond1: pH: 6.7; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCsample_1not availablecond1
HNCOsample_1not availablecond1
HNCACBsample_1not availablecond1
HNCAsample_1not availablecond1
HN(CO)CAsample_1not availablecond1
CBCACONHsample_1not availablecond1
HC(CO)NHsample_1not availablecond1

Software:

PRONTO v20020517, Carlsberg A/S - assignment

NMR spectrometers:

  • Varian UnityInova 800 MHz

Related Database Links:

BMRB 6007
PDB
DBJ BAG35029
EMBL CAA45860 CAA50265 CAG46778 CAG46806 CAH18147
GenBank AAH11024 AAX36546 AAX37102 EAW64735 EAW64736
REF NP_003269
SWISS-PROT P05452

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks