BMRB Entry 6007

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PDB ID: 1rjh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6007
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N resonance assignment of the Plasminogen Kringle 4 Binding Calcium Free Form of the C-type Lectin-like Domain of Tetranectin

Deposition date:

2003-11-14

Original release date:

2004-07-08

Authors:

Nielbo, Steen; Thomsen, Jens; Jensen, Peter; Graversen, Jonas; Etzerodt, Michael; Poulsen, Flemming; Thogersen, Hans

Citation:

Citation: Nielbo, Steen; Thomsen, Jens; Jensen, Peter; Graversen, Jonas; Etzerodt, Michael; Poulsen, Flemming; Thogersen, Hans. "Structure of the Plasminogen Kringle 4 Binding Calcium Free Form of the C-type Lectin-like Domain of Tetranectin" Biochemistry 43, 8636-8643 (2004).

Assembly members:

Assembly members:
CTLD of tetranectin, polymer, 137 residues, 15122 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CTLD of tetranectin: ALQTVCLKGTKVHMKCFLAF TQTKTFHEASEDCISRGGTL STPQTGSENDALYEYLRQSV GNEAEIWLGLNDMAAEGTWV DMTGARIAYKNWETEITAQP DGGKTENCAVLSGAANGKWF DKRCRDQLPYICQFGIV

Data sets:

assigned_chemical_shifts
- apotn3_set1

Data type	Count
13C chemical shifts	454
15N chemical shifts	115
1H chemical shifts	681

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	tn3 calcium free form	1

Entities:

Entity 1, tn3 calcium free form 137 residues - 15122 Da.

1

ALA

LEU

GLN

THR

VAL

CYS

LEU

LYS

GLY

THR

2

LYS

VAL

HIS

MET

LYS

CYS

PHE

LEU

ALA

PHE

3

THR

GLN

THR

LYS

THR

PHE

HIS

GLU

ALA

SER

4

GLU

ASP

CYS

ILE

SER

ARG

GLY

THR

LEU

5

SER

THR

PRO

GLN

THR

GLY

SER

GLU

ASN

ASP

6

ALA

LEU

TYR

GLU

TYR

LEU

ARG

GLN

SER

VAL

7

GLY

ASN

GLU

ALA

GLU

ILE

TRP

LEU

GLY

LEU

8

ASN

ASP

MET

ALA

GLU

GLY

THR

TRP

VAL

9

ASP

MET

THR

GLY

ALA

ARG

ILE

ALA

TYR

LYS

10

ASN

TRP

GLU

THR

GLU

ILE

THR

ALA

GLN

PRO

11

ASP

GLY

LYS

THR

GLU

ASN

CYS

ALA

VAL

12

LEU

SER

GLY

ALA

ASN

GLY

LYS

TRP

PHE

13

ASP

LYS

ARG

CYS

ARG

ASP

GLN

LEU

PRO

TYR

14

ILE

CYS

GLN

PHE

GLY

ILE

VAL

Samples:

sample_1: CTLD of tetranectin, [U-13C; U-15N], 0.6 mM; EDTA 2 mM; NaN3 1 mM; D2O 10%

cond1: pH: 6.7; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
15N-HSQC	not available	not available	not available
HNCO	not available	not available	not available
HNCACB	not available	not available	not available
HNCA	not available	not available	not available
HN(CO)CA	not available	not available	not available
CBCACONH	not available	not available	not available
HNCACO	not available	not available	not available
15N-NOESY-HSQC	not available	not available	not available
15N-TOCSY-HSQC	not available	not available	not available
13C-NOESY-HSQC	not available	not available	not available
HCCH-TOCSY	not available	not available	not available

Software:

PRONTO v20020517, Carlsberg A/S - assignment

NMR spectrometers:

Varian UnityInova 800 MHz

BMRB	6008
PDB	1HTN 1RJH 1TN3
DBJ	BAG35029
EMBL	CAA45860 CAA50265 CAG46778 CAG46806 CAH18147
GenBank	AAH11024 AAX36546 AAX37102 EAW64735 EAW64736
REF	NP_003269
SWISS-PROT	P05452